Regulation of perinatal respiration-related activity and Cl- homeostasis : searching for the factors of sudden infant death syndrome in the brain
| Project/Area Number |
21791046
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
OKABE Akihito Hyogo College of Medicine, 医学部, 講師 (10313941)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 乳幼児突然死症候群 / KCC2 / 細胞内Cl^-濃度 / GABA_A受容体 / 舌下神経核 / 細胞内C1^-濃度 / グリシン受容体 |
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| Research Abstract |
Cation-chloride cotransporters are considered to play critical roles in the regulation of intracellular chloride ion concentration ([Cl^-]_i), and hence in the control of neuronal function. First, I tested the immunoreactivity of KCC2 using KCC2 antibody in developmental mouse medulla. Immunoreactivity of KCC2 in hypoglossal nucleus at P0 was higher than that at P7, while that of vagus nerve nucleus did not change during same period. Since developmental changes in expression pattern of KCC2 associated with respiration-related rhythmic activity (RRA) in medulla are not yet well understood, I recorded RRA extracellulary from respiration-related neurons in medullary slice preparation obtained from mice of P0-7. Under these conditions, perfusion of 100μM GABA-containing ACSF transiently increased the frequency of RRA. The KCC2 inhibitor furosemide also increased the frequency of RRA. Thus Cl^- homeostasis likely regulates the activity of motor output in early postnatal stage.
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Report
(3 results)
Research Products
(14 results)
