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Analysis of the function of Kallikrein 8 in skin wound healing

Research Project

Project/Area Number 21791054
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionAsahikawa Medical College

Principal Investigator

KISHIBE Mari  旭川医科大学, 医学部, 特任助教 (90431410)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords皮膚生理学 / カリクレイン8 / 創傷治療 / カリクレイン6 / Protease-activated receptor 2 / 創傷治癒 / 表皮リモデリング / 乾癬
Research Abstract

Kallikrein-related peptidase 8 (KLK8) is believed to be involved in the maintenance of skin homeostasis and pathogenesis of inflammatory skin diseases. Although previous studies have shown that KLK8 is expressed around incisional wounds, the exact role of KLK8 in wound healing remains obscure. In the present study, we compared wound healing in wild type (WT) and Klk8 gene-disrupted (Klk8^<-/->) mouse skin. Wound healing in Klk8^<-/-> mice was hampered with defective keratinocyte proliferation, differentiation and migration in the early stages of wound healing. Compared with the prominent induction of Klk6 and PAR2 mRNA and protein in WT mice after wounding, a much lower increase was observed in Klk8^<-/-> skin. After skin wounding in WT mice, increased Klk6 was detected from the upper stratum spinosum to the stratum corneum. Moreover, in WT mice, Klk6 protein was processed. PAR2 was diffusely expressed in the cytoplasm of the stratum spinosum at days 7 post-wounding in WT. These results suggest that Klk8 is involved in the proliferation and migration of keratinocytes through the upregulation and activation of Klk6 in the early stages of wound healing, and possibly in keratinocyte differentiation associated with the upregulation and activation of PAR2 in the late stages of wound healing.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2012 2011 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Kallikrein-related peptidase 8-dependent skin wound healing is associated with upregulation of kallikrein-related peptidase 6 and PAR22012

    • Author(s)
      Kishibe M, Bando Y, Tanaka T, Ishida-Yamamoto A, Iizuka H, Yoshida S
    • Journal Title

      J Invest Dermatol

      Volume: (印刷中) Issue: 6 Pages: 1717-1724

    • DOI

      10.1038/jid.2012.18

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kallikrein-Related Peptidase 8-Dependent Skin Wound Healing Is Associated with Upregulation of Kallikrein-Related Peptidase 6 and PAR22012

    • Author(s)
      Mari Kishibe
    • Journal Title

      Journal of Investigative Dermatology

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 皮膚創傷治癒におけるKallikrein-related peptidase-8 (KLK8)の機能解析2011

    • Author(s)
      岸部麻里、山本明美、飯塚一、板東良雄、吉田成孝
    • Organizer
      第回日本皮膚科学会北海道地方会
    • Place of Presentation
      日本、札幌
    • Year and Date
      2011-06-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 皮膚創傷治癒におけるKallikrein-related peptidase-8(Klk8)の機能解析2011

    • Author(s)
      岸部麻里
    • Organizer
      第日本皮膚科学会北海道地方会
    • Place of Presentation
      札幌
    • Year and Date
      2011-06-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kallikrein-related peptidase 8 Is Involved In Skin Wound Healing Through Upregulation of Klk6 and Protease activated receptor2011

    • Author(s)
      Kishibe M, Igawa S, Ishida-Yamamoto A, Iizuka H.
    • Organizer
      36th Annual meeting of the Japan Society of Investigative Dermatology
    • Place of Presentation
      Japan, Kyoto
    • Related Report
      2010 Final Research Report
  • [Presentation] Kallikrein-related peptidase 8 Is Involved In Skin Wound Healing Through Upregulation of Klk6 and Protease activated receptor 22011

    • Author(s)
      Mari Kishibe
    • Organizer
      The 36^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
    • Place of Presentation
      日本(京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kallikrein 8 Is Involved In Skin Wound Healing2009

    • Author(s)
      岸部麻里
    • Organizer
      第34回日本研究皮膚科学会
    • Place of Presentation
      日本(福岡)
    • Year and Date
      2009-12-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Kallikrein 8 Is Involved In Skin Wound Healing2009

    • Author(s)
      Kishibe M, Bando Y, Ishida-Yamamoto A, Yoshida S, Iizuka H.
    • Organizer
      The 34th Annual Meeting of the Japan Society of Investigative Dermatology
    • Place of Presentation
      Japan, Fukuoka
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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