| Project/Area Number |
21791068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
AOKI Rui University of Yamanashi, 医学部附属病院, 助教 (10377541)
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| Research Collaborator |
KAWAMURA Tatsuyoshi 山梨大学, 医学部附属病院, 講師 (70262657)
SHIMADA Shinji 山梨大学, 医学部附属病院, 教授 (10114505)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 皮膚感染症 / HSV / mast cell / innate immunity / ウイルス / マスト細胞 |
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| Research Abstract |
Mast cells are not only effector cells in allergic responses, but are also initiator and regulator cells in both innate and adaptive immune responses. They are known as important players in host defense against bacteria, however, their role in viral infections is largely unknown. We have reported that intradermal injection with herpes simplex virus 2 (HSV-2) into mast cell deficient Kit^<W/W-v> mice lead to increased clinical severity and mortality with elevated levels of virus titers in HSV-infected skins. Moreover, the severe mortality in Kit^<W/W-v> mice was completely recovered by intradermal reconstitution with bone marrow-derived mast cells (BMMCs) which were derived from wild-type (WT), but not TNF^<-/-> or IL-6^<-/->, mice. Here, we show that HSV did not directly induce these cytokines production, whereas supernatants from HSV-infected keratinocytes, Pam 212, induced TNF-α and IL-6 production by BMMCs. Thus, our findings suggest that mast cells are involved in host defense at HSV-infected sites through TNF-α and IL-6 production.
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