Regulatory factor on binding of Desmoglein3 and p120ctn during blister formation in pemphigus

• Project/Area Number: 21791070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Gifu University
• Principal Investigator: SHU En 岐阜大学, 医学系研究科, 非常勤講師 (60444288)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 皮膚病理学 / 天疱瘡 / デスモグレイン3 / P120カテニン / p120カテニン

Research Abstract

In order to elucidate the signal pathway of blister formation in pemphigus, we examined the phosphorylation of pl2Octn in keratinocytes treated with pemphigus-IgG.(1) We performed immunofluorescence staining with DJM-1 cells treated with pemphigus-IgG, however, phospho-p120ctn were not observed.(2) We stimulated normal human keratinocytes and subjected those cells to immunoprecipitation. There are no significant differences in phosphorylation of p120ctn between untreated cells, cells treated with pemphigus and normal human IgG.