| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
>To study the role of ectopic epidermal auto-antigen expression, K5-Cre x CAG-CAT-EGFP double transgenic mice that express enhanced green fluorescence protein (GFP) under the control of keratin 5 promoter were employed. Unexpectedly, GFP^<high> cells distinct from migratory DC were found not only in skin-draining LN, but also in mesenteric LN, spleen, peripheral blood and bone marrow, indicating that bone marrow-derived circulating cells express keratin 5. Interestingly, GFP^<high> cells strongly expressed MHC class II and were enriched from selected CD45R (B220) + and IgD- population. GFP^<high> cells crosspresented ovalbumin (OVA) as well as immature B cells and pDC in vitro, whereas GFP^<high> cells and immature B cells, but not pDC, presented OVA to CD4 T cells. These results indicate that immature B cell-like circulating lymphoid cell is an antigen-presenting cell having a unique characteristic to express ectopic self-antigen.
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