Analysis of allergen-specific IgE repertoire in wheat allergy and its application for the diagnosis
| Project/Area Number |
21791078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hiroshima University |
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Principal Investigator |
MATSUO Hiroaki Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (60346385)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 小麦アレルギー / 検査 / アレルゲン / IgE抗体 / リコンビナント / 小麦アレギー |
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| Research Abstract |
In this study, we tried to produce the recombinant wheat allergens in Escherichia coli to establish a new in vitro method for accurate diagnosis of wheat allergy. We succeeded in producing the water-soluble wheat allergens as fusion proteins with glutathione-S-transferase by means of a co-expression of chaperon plasmid and pGEX-6p-1. The recombinant water-insoluble allergens were expressed using a pET or pCold system. The IgE-binding to some of the recombinant allergens were detected indicating that these recombinant proteins can be applied to the measuring of allergen-specific IgE antibodies.
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Report
(3 results)
Research Products
(18 results)
