Role of pyrin-related molecules in vascular endothelial cells and their genetic mutations in vasculitis syndromes
| Project/Area Number |
21791087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
NAKAMURA Tomoyuki Wakayama Medical University, 医学部, 博士研究員 (10405459)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Pyrin / 血管内皮細胞 / 血管炎症候群 |
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| Research Abstract |
Expression of the inflammasome components, NLRP3, ASC and pro-caspase-1, as well as pyrin, in F-2 murine vascular endothelial cells was confirmed with RT-PCR. IL-1β production in culture media of F-2 cells after stimulation with R837, one of NLRP3 ligands, was detected by ELISA, suggesting the presence of functional inflammasome in F-2. Pretreatment with LPS significantly increased IL-1β production of R837-stimulated F-2 cells. By FACS analyses, it was revealed that F-2 cells producing IL-1β can bind Annexin V but show no change after labeling with mitotracker or lysotracker. Genetic analysis of the cases, who showed fever of unknown origin, purpura or nodular erythemas as the main symptom, and was suspected to be suffering from an autoinflammatory syndrome with vasculopathy, has not revealed any mutation in these molecules.
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Report
(3 results)
Research Products
(23 results)
