| Project/Area Number |
21791089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KANAZAWA Nobuo Wakayama Medical University, 医学部, 講師 (90343227)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | NOD2 / 肉芽腫 / NF-κB |
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| Research Abstract |
Human monocytic THP-1 cells expressing early-onset sarcoidosis/Blau syndrome-associated mutant NOD2 were applied for analysis of their phenotype without stimulation or when stimulated with PMA, which induces their differentiation into macrophage-like cells. No significant difference was observed among phenotype of THP-1 derivatives without stimulation. After PMA addition, mutant THP-1 cells were attached to the culture plate for a long period without proliferation, while control cells were attached only transiently. By analysis of surface adhesion molecules, surface ICAM-1 expression level was shown to be correlated with capacity of the cells to attach the plate. Furthermore, PDGF-B was discovered as a cytokine which is specifically produced by mutant THP-1 cells after PMA stimulation. Actually, it was confirmed that ICAM-1 and PDGF-B were expressed on mutant Nod2-expressing multinucleated giant cells in the granulomatous skin lesion of an early-onset sarcoidosis patient.
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