| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Metastasis formation is responsible for most cancer deaths. Brain metastases, most often with melanomas as with lung and breast carcinomas, induce a high morbidity and mortality. Our analysis of many human cancer cell lines using bio-luminescent imaging (BLI) technology, has demonstrated cancer cell-type dependent metastasis to specific organs of NOD/SCID mice. Herein we established brain-tropic melanoma cell lines (SK-MEL-28-luc-br2 and Mewo-luc-br2) after 3 cycles of sequential intra-cardiac (i.e.) inoculation into mice, and determined the kinetics of brain accumulation of melanoma cells after i.e. injection. While both the brain-tropic and the low-metastatic melanoma (SK-MEL-2-luc) cells were present to similar degrees in the brain 0.5-5 hrs after injection, brain-tropic melanoma cells showed a significant increase in tumor-derived photons at the late phase (after 15-20 days). Further a comprehensive transcriptome analysis showed a significant increase (3-6 fold) of TGF-beta receptor II in brain-tropic melanoma and breast carcinoma (MDA-MB-231-luc-brl) cells. The BLI-mediated in vivo imaging should promote identification of potential molecular markers for cancer metastasis.
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