Analysis of morphology and adhesion molecules of skin dendritic cells

Research Project

Project/Area Number	21791094
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Dermatology
Research Institution	Keio University
Principal Investigator	OUCHI Takeshi Keio University, 医学部, 助教 (30528419)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	皮膚樹状細胞 / 形態 / 接着分子 / 免疫電顕 / 蛍光抗体法
Research Abstract	We attempted to analyze ultrastructural morphology and localization of adhesion molecules on skin DCs by using immunofluorescent staining and immuno electron microscopy. Precise morphology of skin dendritic cells by immuno electron microscopy could not be observed because of the degeneration of specimen due to fixation processes and poor accessibility of antibody. Immunofluorescence staining revealed accumulation of langerin+ dermal dendritic cells around hair follicles and their interaction with epidermal Langherhans cells in the process of emigration. These findings suggest the possibility of interaction among skin dendritic cell subsets.

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