Development of a New Model for Atopic Dermatitis Using Filaggrin Mutant Mice

• Project/Area Number: 21791095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: KAWASAKI Hiroshi Keio University, 医学部, 研究員(非常勤) (70445344)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 皮膚バリア / アトピー性皮膚炎 / フィラグリン

Research Abstract

To better understand the functional properties of filaggrin and to develop a new model for elucidating the pathogenesis of atopic dermatitis, flaky tail mice, which have mutant filaggrin and its expression is significantly reduced, were utilized. This study exhibited enhanced outside-to-inside permeability in flaky tail mice, however it indicated filaggrin deficiency itself was not affected with increased transepidermal water loss. Flaky tail mice showed enhanced percutaneous immune response, as compared with wild-type mice.

Research Products

• [Journal Article] SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing (2011)
  • Author(s): Matsui T
  • Journal Title: EMBO Molecular Medicine Volume: (in press)
  • Peer Reviewed
• [Journal Article] Flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with Dermatophagoides pteronyssinus extract. (2010)
  • Author(s): Moniaga CS, Egawa G, Kawasaki H, Hara-Chikuma M, Honda T, Tanizaki H, Nakajima S, Otsuka A, Matsuoka H, Kubo A, Sakabe J, Tokura Y, Miyachi Y, Amagai M, Kabashima K
  • Journal Title: Am J Pathol 176(5) Pages: 2385-93
  • Peer Reviewed
• [Journal Article] Flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with Dermatophagoides pteronyssinus extract. (2010)
  • Author(s): Moniaga CS
  • Journal Title: American Journal of Pathology Volume: 176 Pages: 2385-93
  • Peer Reviewed
• [Journal Article] Tail Mouse Denotes Human Atopic Dermatitis in the Steady State and by Topical Application with Dermatophagoides pteronyssinus Extract. (2010)
  • Author(s): Moniaga CS, et al.
  • Journal Title: American Journal of Pathology (in press)
  • Peer Reviewed
• [Journal Article] SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing.
  • Author(s): Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M
  • Journal Title: EMBO Mol Med. (in press)
  • Peer Reviewed
• [Presentation] Filaggrin-null mice exhibit altered skin barrier formation and enhanced percutaneousi mmune responses. (2010)
  • Author(s): Kawasaki H
  • Organizer: The 35^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Wakayama, Japan.
  • Year and Date: 2010-12-03
• [Presentation] Filaggrin-null mice exhibit altered skin barrier formation and enhanced percutaneous immune responses. (2010)
  • Author(s): 川崎洋
  • Organizer: The 35^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 和歌山県民文化会館(和歌山県)
  • Year and Date: 2010-12-03
• [Presentation] Filaggrin knockout mice as a tool for understanding percutaneous antigen exposure in barrier-disrupted skin. (2010)
  • Author(s): Kawasaki H
  • Organizer: the 40^<th> Annual Meeting of European Society for Dermatological Research
  • Place of Presentation: Helsinki, Finland.
  • Year and Date: 2010-09-10
• [Presentation] Filaggrin knockout mice as a tool for understanding percutaneous antigen exposure in barrier-disrupted skin. (2010)
  • Author(s): 川崎洋
  • Organizer: The 40^<th> Annual Meeting of European Society for Dermatological Research
  • Place of Presentation: Helsinki (Finland)
  • Year and Date: 2010-09-10
• [Presentation] Filaggrin Knockout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis. (2010)
  • Author(s): Kawasaki H
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, Japan.
  • Year and Date: 2010-08-25
• [Presentation] Filaggrin Knockout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis. (2010)
  • Author(s): 川崎洋
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテル、神戸国際展示場(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] Filaggrin Knocout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis Filaggrin Knocout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis. (2009)
  • Author(s): 川崎洋
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] Filaggrin Knocout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis Filaggrin Knocout Mice as a Tool for Understanding the Pathogenesis of Atopic Dermatitis. (2009)
  • Author(s): 川崎洋
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-12-03
• [Presentation] アトピー性皮膚炎病態解明のためのフィラグリン欠失マウスの作製. (2009)
  • Author(s): 川崎洋
  • Organizer: 第16回分子皮膚科学フォーラム
  • Place of Presentation: 札幌
  • Year and Date: 2009-11-21
• [Presentation] アトピー性皮膚炎病態解明のためのフィラグリン欠失マウスの作製 (2009)
  • Author(s): 川崎洋
  • Organizer: 第16回分子皮膚科学フォーラム
  • Place of Presentation: 札幌グランドホテル(北海道)
  • Year and Date: 2009-11-21
• [Book] Loss-of-function mutations within the filaggrin gene predispose to atopic dermatitis. 41 (2011)
  • Author(s): Kawasaki H, Kubo A, Sasaki T, Amagai M.
  • Publisher: KARGER. Curr Probl Dermatol.(in press)
• [Remarks] ホームページ等