The analyses of mechanism to induce pemphigus using desmoglein 3-GFP transgenic mice

• Project/Area Number: 21791096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: YOSHIDA Kazue Keio University, 医学部, 研究員 (70383776)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 天疱瘡 / デスモグレイン

Research Abstract

Pemphigus vulgaris is serious autoimmune blistering disease induced by autoantibodies against desmoglein3 (Dsg3). To make Dsg3 visible, we made a Dsg3-GFP transgenic mouse. When we injected fluorescent labeling anti-Dsg3 antibody into Dsg3-GFP mouse ear skin, we have succeeded in observing the movement of Dsg3 and anti-Dsg3 antibody in vivo. Our observation would be useful to develop the disease-specific therapy to inhibit bulla formation in pemphigus vulgaris in the future.