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Roles of sphingosine-1-phosphate foreosinophil localization and function in mice

Research Project

Project/Area Number 21791105
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

SUGITA Kazunari  University of Occupational and Environmental Health, Japan, 医学部, 助教 (40412647)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords皮膚炎症 / 再生学 / 好酸球 / S1P / S1P1 / FTY720
Research Abstract

We monitored numerical alternations of eosinophils in IL-5 transgenic mice following FTY720 administration. The number of circulating eosinophils was significantly decreased in IL-5 transgenic mice treated by FTY720,suggesting that eosinophils are S1P-S1P1 sensitive. Accordingly, eosinophils showed a chemotactic response to S1P through a transwell assay. Additionally,eosinophils accumulated in the bone marrow, when S1P mediated signaling was disrupted by FTY720 treatment. We then showed the increment of cutaneous eosinophils using a murine atopic dermatitis model induced by repeated hapten application. FTY720 administration before the final challenge decreased the number of cutaneous eosinophils along with reduction of the ear swelling response, of the late phase reaction. These findings suggest that S1P-S1P1 signaling regulates the egress of eosinophils from the bone marrow and affects cutaneous eosinophil localization and resultant late phase reaction.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] FTY720 regulates bone marrow egress of eosinophils and modulates late-phase skin reaction in mice.2010

    • Author(s)
      Sugita K, Kabashima K, Sakabe J, Yoshiki R, Tanizaki H, Tokura Y.
    • Journal Title

      Am J Pathol. 177

      Pages: 1881-1887

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] FTY720 regulates bone marrow egress of eosinophils and modulates late-phase skin reaction in mice2010

    • Author(s)
      Kazunari Sugita
    • Journal Title

      The American Journal of Pathology

      Volume: 177 Pages: 1881-1887

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sphingosine-1phosphate regulates cutaneous localization and bonemarrow egress of eosinophils.2009

    • Author(s)
      杉田和成
    • Organizer
      日本研究皮膚科学会第34回年次学術大会・総会
    • Place of Presentation
      福岡市
    • Year and Date
      2009-12-05
    • Related Report
      2010 Final Research Report
  • [Presentation] Sphingosine-1phosphate regulates cutaneous localization and bone marrow egress of eosinophils2009

    • Author(s)
      杉田和成
    • Organizer
      日本研究皮膚科学会第34回年次学術大会・総会
    • Place of Presentation
      福岡市(JALリゾートシーホークホテル福岡)
    • Year and Date
      2009-12-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Sphingosine-1phosphate regulates cutaneous localization and bone marrow egress of eosinophils.2009

    • Author(s)
      杉田和成
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪市
    • Year and Date
      2009-12-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Sphingosine-1phosphate regulates cutaneous localization and bone marrow egress of eosinophils2009

    • Author(s)
      杉田和成
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪市(大阪国際会議場)
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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