Detection of mitochondrial DNA defects in GABAergic neurons of model mice for bipolar disorder

• Project/Area Number: 21791157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KUBOTA Mie 独立行政法人理化学研究所, 精神疾患動態研究チーム, 専門職研究員 (90344035)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Mitochondria / polymerase gamma / mitochondrial DNA / bipolar disorder / GFP / mitochondria / polymeraseγ / mtDNA

Research Abstract

We detected the distribution of mtDNA deficient neurons with loss of mitochondrial proteins in transgenic mice expressing neuron-specific mutant mitochondria DNA polymerase by immunohistochemistry with antibodies against mtDNA-encoded or nuclear-encoded mitochondrial proteins.

Research Products

• [Presentation] 変異Polg1が脳内に発現するマウスにおけるミトコンドリアDNA欠失蓄積細胞の組織学的検討 (2011)
  • Author(s): 窪田-坂下美恵
  • Organizer: 第33回日本生物学的精神医学会
  • Place of Presentation: 東京都(港区台場)
  • Year and Date: 2011-07-15
• [Presentation] Histochemical detection of neurons accumulating mitochondrial DNA deletions in mice with mutant Polg1 gene (2011)
  • Author(s): Mie Kubota-Sakashita
  • Organizer: 8^<th> World Congress of Neuroscience(International Brain Research Organization)
  • Place of Presentation: Italy(Florence)
  • Year and Date: 2011-05-22
• [Presentation] Histochemical detection of neurons accumulating mitochondria 1 DNA deletions in mice with mutant Polg1 gene (2011)
  • Author(s): Mie Kubota-Sakashita
  • Organizer: 8^<th> World Congress of Neuroscience (International Brain Research Organization)
  • Place of Presentation: Italy (Florence)
  • Year and Date: 2011-05-22
• [Presentation] 異常なミトコンドリアDNAが脳内に蓄積するマウスにおけるドーパミン神経系障害に関する検討 (2009)
  • Author(s): 窪田美恵
  • Organizer: 日本生物学的精神医学会
  • Place of Presentation: 京都市・国立京都国際会館
  • Year and Date: 2009-04-24
• [Remarks]
  • URL: http://www.brain.riken.jp/jp/faculty/details/22