Establishment of tracking method for induced pluripotent stem cells using 64-Cu positron emitting isotope
| Project/Area Number |
21791171
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
NAKAJIMA Takahito Gunma University, 大学院・医学系研究科, 寄附講座教員 (70375559)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | iPS細胞 / 64-Cu / ポジトロン核種 / 放射線 / 再生医学 / 細胞・組織 |
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| Research Abstract |
Induced pluripotent stem cells (iPSCs) Labeling by 64-Cu positron emitting isotopes is a technical success with using PTSM material as a binding material. And positron emission tomography (PET) images which indicate iPSC distributions were acquired successfully. Two administration pathways were used ; 1) intravenous injection via tail vein, 2) left ventricle injection directly. There was significant difference between each distribution. Only lung distribution was seen by intravenous injection. On the other hand, the distributions to brain, kidneys, and transplanted tumors were described. Since half-life for 64-Cu is 13 hours, PET images could be acquired at 24 hours after labeled cells injection. Comparison with images for 18F-FDG labeled iPSCs, 64-Cu labeled image has less signal-to-noise ratio than that of 18F-FDG within 6 hour after positron-labeled cells administration.
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Report
(3 results)
Research Products
(3 results)
