Development of the high-sensitive imaging method for HIF-1-active hypoxic tumors based on the metabolic trapping approach
Project/Area Number |
21791187
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Radiation science
|
Research Institution | Kyoto University |
Principal Investigator |
UEDA Masashi Kyoto University, 医学研究科, 助教 (40381967)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 放射性医薬品 / 分子イメージング / 低酸素 / HIF-1 / 代謝捕捉 |
Research Abstract |
Hypoxia-inducible factor-1 (HIF-1) plays an important role in malignant tumor progression and in the development of resistance to radiotherapy. I designed a novel fusion protein (POTK) consisting of a protein transduction domain, herpes simplex virus type 1 thymidine kinase, and an essential part of the oxygen-dependent degradation domain of HIF-1 α that confers the same oxygen-dependent regulation as HIF-1α on POTK. I demonstrated that POTK and [^<123>I]FIAU, which is metabolized by POTK and trapped intracellularly, is potential probes for high-sensitive imaging of HIF-1-active regions in tumors.
|
Report
(3 results)
Research Products
(16 results)
-
[Journal Article] PET Imaging of Hypoxia-Inducible Factor-1-Active Tumor Cells with Pretargeted Oxygen-Dependent Degradable Streptavidin and a Novel ^<18>F-Labeled Biotin Derivative2011
Author(s)
Kudo T, Ueda M, Konishi H, Kawashima H, Kuge Y, Mukai T, Miyano A, Tanaka S, Kizaka-Kondoh S, Hiraoka M, Saji H
-
Journal Title
Mol Imaging Biol
Volume: (印刷中)
NAID
Related Report
Peer Reviewed
-
[Journal Article] Rapid detection of hypoxia-inducible factor-l-active tumours : pretargeted imaging with a protein degrading in a mechanism similar to hypoxia-inducible factor-lalpha.2010
Author(s)
Ueda M, Kudo T, Kuge Y, Mukai T, Tanaka S, Konishi H, Miyano A, Ono M, Kizaka-Kondoh S, Hiraoka M, Saji H.
-
Journal Title
Eur J Nucl Med Mol Imaging. 37
Pages: 1566-74
Related Report
Peer Reviewed
-
[Journal Article] Rapid detection of hypoxia-inducible factor-1-active tumours : pretargeted imaging with a protein degrading in a mechanism similar to hypoxia-inducible factor-1alpha2010
Author(s)
Ueda M, Kudo T, Kuge Y, Mukai T, Tanaka S, Konishi H, Miyano A, Ono M, Kizaka-Kondoh S, Hiraoka M, Saji H
-
Journal Title
Eur J Nucl Med Mol Imaging
Volume: 37
Pages: 1566-74
NAID
Related Report
Peer Reviewed
-
[Journal Article] PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel 18^F-labeled biotin derivative.
Author(s)
Kudo T, Ueda M, Konishi H, Kawashima H, Kuge Y, Mukai T, Miyano A, Tanaka S, Kizaka-Kondoh S, Hiraoka M, Sail H.
-
Journal Title
Mol Imaging Biol. (in press)
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
[Presentation] Pretargeted PET imaging of hypoxia-inducible factor-1-active tumors with an oxygen-dependent degradable streptavidin and a ^<18>F-labeled biotin derivative2010
Author(s)
Ueda M, Kudo T, Konishi H, Kawashima H, Kuge Y, Mukai T, Ono M, Kizaka-Kondoh S, Hiraoka M, Saji H
Organizer
2010 World Molecular Imaging Congress
Place of Presentation
Kyoto, Japan
Year and Date
2010-09-08
Related Report
-
-
-
-
-
-