Involvement of the microenvironment on the radiosensitivity

• Project/Area Number: 21791190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Kyoto University
• Principal Investigator: KASHINO Genro Kyoto University, 原子炉実験所, 助教 (00437287)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 微小環境 / バイスタンダー効果 / ミトコンドリア / 活性酸素 / 突然変異 / 53BP1 / 放射線感受性 / 分泌因子 / DNA二重鎖切断

Research Abstract

It has been suggested that tumor microenvironment is important for the therapy. In this study, we examined the effects of secreted factors from irradiated cells on the treated cells to check the microenvironment after irradiation. The results suggest that membrane activity of mitochondria was reduced, and the level of reactive oxygen species was increased by the treatments of the secreted factors. Also, mutation fraction was increased in cells treated with secreted factors. However, these effects were not affected in radiosensitivity indicated by the foci formation of 53BP1 after X-irradiation.

Research Products

• [Journal Article] Usefulness of hexamethylenetetramine as an adjuvant to radiation and cisplatin in the treatment of solid tumors : its independency of p53 status (2010)
  • Author(s): Masunaga S, Tano K, Nakamura J, Watanabe M, Kashino G, Takahashi A, Tanaka H, Suzuki M, Ohnishi K, Kinashi Y, Liu Y, Ohnishi T, Ono K.
  • Journal Title: J.Radiat.Res. 51 Pages: 27-35
  • NAID: 10025914038
  • Peer Reviewed
• [Journal Article] Influence of manipulating hypoxia in solid tumors on the radiation dose-rate effect in vivo, with reference to that in the quiescent cell population. (2010)
  • Author(s): Masunaga S, Hirayama R, Uzawa A, Kashino G, Takata T, Tanaka H, Suzuki M, Kinashi Y, Liu Y, Koike S, Ando K, Ono K.
  • Journal Title: Jap.J.Raiol. 28 Pages: 132-142
  • NAID: 120002511344
  • Peer Reviewed
• [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide; possible facilitation of DNA double-strand break repair. (2010)
  • Author(s): Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
  • Journal Title: J.Radiat.Res. 51 Pages: 733-740
  • NAID: 10026964239
  • Peer Reviewed
• [Journal Article] Ascorbic acid 2-glucocide reduces micronucleus induction in distant splenic T lymphocytes following head irradiation. (2010)
  • Author(s): Kinashi Y, Tanaka H, Masunaga S, Suzuki M, Kashino G, Yong L, Takahashi S, Ono K.
  • Journal Title: Mutat.Res. 695 Pages: 69-74
  • Peer Reviewed
• [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide ; possible facilitation of DNA double-strand break repair. (2010)
  • Author(s): Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
  • Journal Title: Journal of Radiation Research Volume: 51 Pages: 733-740
  • NAID: 10026964239
  • Peer Reviewed
• [Journal Article] Inductions of DNA double strand breaks and cellular migrations through the bystander effects in the cells irradiated with slit type microplanar beam of the SPring-8 synchrotron. (2009)
  • Author(s): Kashino G, Kondoh T, Nariyama N, Umetani K, Ohigashi T, Shinohara K, Kurihara A, Fukumoto M, Tanaka H, Maruhashi A, Suzuki M, Kinashi Y, Liu Y, Masunaga S, Watanabe M, Ono K
  • Journal Title: Int.J Radiat.Oncol.Biol.Physic. 74 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Spectromicroscopic film dosimetry for high-energy microbeam from synchrotron radiation. (2009)
  • Author(s): Nariyama N, Ohigashi T, Umetani K, Shinohara K, Tanaka H, Maruhashi A, Kashino G, Kurihara A, Kondob T, Fukumoto M, Ono K
  • Journal Title: Appl.Radiat.Isot. 67 Pages: 155-159
  • Peer Reviewed
• [Journal Article] Watanabe M, Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities. (2009)
  • Author(s): Yoshikawa T, Kashino G, Ono K
  • Journal Title: J.Radiat.Res. 50 Pages: 151-160
  • Peer Reviewed
• [Journal Article] Usefulness of hexamethylenetetramine in combination with chemotherapy using free and pegylated liposomal doxorubicin in vivo, referring to the effect on quiescent cells (2009)
  • Author(s): Masunaga S, Kono K, Nakamura J, Tano K, Yoshida H, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Liu Y, Ono K.
  • Journal Title: Oncol.Rep. 21 Pages: 1307-1312
  • Peer Reviewed
• [Journal Article] Nakamura J : Evaluation of the potential of hexamethylenetetramine, compared with tirapazamine, as a combined agent with {gamma}-irradiation and cisplatin treatment in vivo. (2009)
  • Author(s): Masunaga S, Tano K, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Ono K
  • Journal Title: Br.J.Radiol. 82 Pages: 392-400
  • Peer Reviewed
• [Journal Article] The effect of post-irradiation tumor oxygenation status on recovery from radiation-induced damage in vivo : with reference to that in quiescent cell populations. (2009)
  • Author(s): Masunaga SI, Hirayama R, Uzawa A, Kashino G, Suzuki M, Kinashi Y, Liu Y, Koike S, Ando K, Ono K
  • Journal Title: J.Cancer Res.Clin.Oncol. 135 Pages: 1109-11016
  • NAID: 120001624124
  • Peer Reviewed
• [Journal Article] Impact of accelerator-based boron neutron capture therapy(AB-BNCT)on the treatment of multiple liver tumors and malignant pleural mesothelioma. (2009)
  • Author(s): Suzuki M, Tanaka H, Sakurai Y, Kashino G, Yong L, Masunaga S, Kinashi Y, Mitsumoto T, Yajima S, Tsutsui H, Sato T, Maruhashi A, Ono K
  • Journal Title: Radiother.Oncol. 92 Pages: 89-95
  • Peer Reviewed
• [Journal Article] A Simple and Rapid Method for Measurement of 10B-para-Boronophenylalanine in the Blood for Boron Neutron Capture Therapy Using Fluorescence Spectrophotometry. (2009)
  • Author(s): Kashino G, Fukutani S, Suzuki M, Liu Y, Nagata K, Masunaga SI, Maruhashi A, Tanaka H, Sakurai Y, Kinashi Y, Fujii N, Ono K
  • Journal Title: J.Radiat.Res. 50 Pages: 377-382
  • NAID: 10025912421
  • Peer Reviewed
• [Journal Article] Gamma-ray irradiation enhanced boron-10 compound accumulation in murine tumors. (2009)
  • Author(s): Liu Y, Nagata K, Masunaga S, Suzuki M, Kashino G, Kinashi Y, Tanaka H, Sakurai Y, Maruhashi A, Ono K.
  • Journal Title: J.Radiat.Res. 50 Pages: 553-557
  • Peer Reviewed
• [Journal Article] Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells. (2009)
  • Author(s): Suzuki K, Kashino G, Kodama S, Watanabe M
  • Journal Title: Mutat.Res. 671 Pages: 33-39
  • NAID: 120006983991
  • Peer Reviewed
• [Journal Article] Inductions of DNA double strand breaks an cellular migrations through the bystander effects in the cells irradiated with slit type microplanar beam of the SPring-8 synchrotron. (2009)
  • Author(s): Kashino G, Kondoh T, Nariyama N, Umetani K, Ohigashi T, Shinohara K, Kurihara A, Fukumoto M, Tanaka H, Maruhashi A, Suzuki M, Kinashi Y, Liu Y, Masunaga S, Watanabe M, Ono K.
  • Journal Title: International Journal of Radiation Oncology, Biology, Physics 74 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities. (2009)
  • Author(s): Yoshikawa T, Kashino G, Ono K, Watanabe M.
  • Journal Title: Journal of Radiation Research 50 Pages: 151-160
  • NAID: 10025911507
  • Peer Reviewed
• [Journal Article] Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells. (2009)
  • Author(s): Suzuki K, Kashino G, Kodama S, Watanabe M.
  • Journal Title: Mutation Research 671 Pages: 33-39
  • NAID: 120006983991
  • Peer Reviewed
• [Presentation] 放射線誘発バイスタンダー効果による突然変異誘発機構 (2010)
  • Author(s): 菓子野元郎、熊谷純、田野恵三、渡邉正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] 放射線誘発バイスタンダー効果による突然変異誘発機構 (2010)
  • Author(s): 菓子野元郎、熊谷純、田野恵三、渡邊正己
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-20
• [Presentation] 放射線照射による分泌性因子を介した突然変異誘発の機構解明 (2010)
  • Author(s): 菓子野元郎、熊谷純、田野恵三、渡邉正己
  • Organizer: 日本組織培養学会第81回大会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-21
• [Presentation] 放射線照射による分泌性因子を介した突然変異誘発の機構解明 (2010)
  • Author(s): 菓子野元郎、熊谷純、田野恵三、渡邉正己
  • Organizer: 日本組織培養学会第81回大会
  • Place of Presentation: 岡山市
  • Year and Date: 2010-05-21
• [Presentation] 放射線により誘導される分泌性因子を介した突然変異生成機構 (2010)
  • Author(s): 菓子野元郎、熊谷純、渡邉正己、小野公二
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-30
• [Presentation] Slow releasing long lived radicals are involved in the bystander mutagenesis response. (2010)
  • Author(s): Genro Kashino, Jun Kumagai, Hiroyuki Kugoh, Mitsuo Oshimura, Keizo Tano, Koji Ono, Masami Watanabe
  • Organizer: 56th Annual Meeting of the Radiation Research Society
  • Place of Presentation: 米国ハワイ州マウイ
• [Presentation] 分泌性因子を介したバイスタンダー効果による突然変異誘発機構 (2009)
  • Author(s): 菓子野元郎、熊谷純、劉勇、鈴木実、木梨友子、増永慎一郎、渡邉正己、小野公二
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-12
• [Presentation] 分泌因子を介したバイスタンダー効果による突然変異誘発機構 (2009)
  • Author(s): 菓子野元郎、熊谷純、劉勇、鈴木実、木梨友子、増永慎一郎、渡邉正己、小野公二
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] SPring-8放射光のスリット状照射による細胞への影響 (2009)
  • Author(s): 菓子野元郎、鈴木雅雄、近藤威、梅谷啓二、成山展照、福本学、劉勇、鈴木実、増永慎一郎、木梨友子、田中浩基、丸橋晃、渡邉正己、小野公二
  • Organizer: 第48回日本医学放射線学会生物部会学術大会
  • Place of Presentation: 富山市
  • Year and Date: 2009-07-10
• [Presentation] 放射線誘発バイスタンダー効果におけるラジカル種の関与 (2009)
  • Author(s): 菓子野元郎、熊谷純、劉勇、木梨友子、鈴木実、増永慎一郎、小野公二、渡邉正己
  • Organizer: 日本保健物理学会第43回研究発表会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-06-03
• [Presentation] Involvement of radical species on radiation induced bystander effects. (2009)
  • Author(s): Genro Kashino
  • Organizer: The 2nd Asian Congress of Radiation Research
  • Place of Presentation: ソウル市
  • Year and Date: 2009-05-19
• [Presentation] Involvement of radical species on radiation induced bystander effects (2009)
  • Author(s): Genro Kashino
  • Organizer: The 2^<nd> Asian Congress of Radiation Research
  • Place of Presentation: ソウル市(KOREA)
  • Year and Date: 2009-05-19
• [Presentation] The role of radicals in bystander effect through the secreted factors from irradiated cells. (2009)
  • Author(s): Genro Kashino, Jun Kumagai, Koji Ono, Masami Watanabe
  • Organizer: 55th Annual Meeting of the Radiation Research Society
  • Place of Presentation: 米国ジョージア州サバンナ
• [Presentation] The role of radicals in bystander effect through the secreted factors from irradiated cells (2009)
  • Author(s): Genro Kashino, Jun Kumagai, Koji Ono, Masami Watanabe
  • Organizer: 55th Annual Meeting of the Radiation Research Society
  • Place of Presentation: 米国ジョージア州サバンナ