Basic Research in Bioimaging for Expression of Hepatocyte Surface Membrane Protein Using Magnetic Resonance Imaging

• Project/Area Number: 21791191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: ONISHI Hiromitsu 大阪大学, 医学系・研究科, 助教 (20452435)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 核磁気共鳴画像(MRI) / 肝細胞特異性造影剤 / ガドキセト酸ナトリウム(Gd-EOB-DTPA) / 肝細胞 / organic anion transporting polypeptide(OATP) / 肝細胞がん / アフリカツメガエル卵母細胞 / アフリカツメガエル / 卵母細胞 / 核磁気共鳴画像 / 膜タンパク質 / ヒト / トランスポーター / Gd-EOB-DTPA

Research Abstract

The main purpose of the present study was to investigate the transporters that mediate the uptake of hepatocyte-specific magnetic resonance imaging contrast agent, gadoxetate disodium. Human organic anion transporting polypeptide(OATP) 2 and 8 have been proven to mediate the uptake of gadoxetate disodium using human cRNA-injected Xenopus laevis oocytes. The other purpose was to evaluate the accuracy of detection of hypervascular hepatocellular carcinoma by gadoxetate disodium-enhanced MR imaging. gadoxetate disodium-enhanced MR imaging revealed significant improvement in sensitivity and diagnostic accuracy for detection of hypervascular hepatocellular carcinoma.

Research Products

• [Journal Article] Hypervascular hepatocellular carcinomas : detection with gadoxetate disodium-enhanced MR imaging and multiphasic multidetector CT (2012)
  • Author(s): Onishi H, Kim T, Imai Y, Hori M, Nagano H, Nakaya Y, Tsuboyama T, Nakamoto A, Tatsumi M, Kumano S, Okada M, Takamura M, Wakasa K, Tomiyama N, Murakami T
  • Journal Title: European Radiology Volume: 22巻 Pages: 845-854
  • Peer Reviewed
• [Journal Article] 肝臓のEOB・プリモビスト造影MRI本検査を有効に活用するための理解すべき課題 (2010)
  • Author(s): 大西裕満, 金東石, 堀雅敏, 巽光朗, 坪山尚寛, 中本篤, 富山憲幸
  • Journal Title: 日獨医報 Volume: 55巻 Pages: 167-178
• [Journal Article] 【肝臓のEOB・プリモビスト造影MRI 本検査を有効に活用するための理解すべき課題】EOB・プリモビスト造影MRI 肝細胞相で非典型的造影所見を示す症例 (2010)
  • Author(s): 大西裕満, 金東石, 堀雅敏, 巽光朗, 坪山尚寛, 中本篤, 富山憲幸
  • Journal Title: 日獨医報 Volume: 55 Pages: 167-178
• [Journal Article] 【消化器画像診断 最近の進歩】早期肝細胞癌と異型結節の鑑別におけるEOB造影MRIの有用性 (2010)
  • Author(s): 今井康陽, 小来田幸世, 関康, 高村学, 大西裕満, 金東石, 岡田真広, 村上卓道
  • Journal Title: Pharma Medica Volume: 28 Pages: 23-27
• [Journal Article] 【Step up MRI 2010再び、3Tである理由】領域別に検証する3T MRIである理由 上腹部領域 肝・胆 3T MRIの現状と臨床的位置づけ (2010)
  • Author(s): 金東石, 堀雅敏, 大西裕満, 富山憲幸
  • Journal Title: INNERVISION Volume: 25 Pages: 24-26
• [Journal Article] Hepatocellular carcinoma : hepatocyte-selective enhancement at gadoxetic acid-enhanced MR imaging--correlation with expression of sinusoidal and canalicular transporters and bile accumulation (2010)
  • Author(s): Tsuboyama T, Onishi H, Kim T, Akita H, Hori M, Tatsumi M, Nakamoto A, Nagano H, Matsuura N, Wakasa K, Tomoda K.
  • Journal Title: Radiology Volume: 255 Pages: 824-833
  • Peer Reviewed
• [Journal Article] Gd-EOB-DTPA-enhanced magnetic resonance images of hepatocellular carcinoma : correlation with histological grading and portal blood flow (2010)
  • Author(s): Kogita S, Imai Y, Okada M, Kim T, Onishi H, Takamura M, Fukuda K, Igura T, Sawai Y, Morimoto O, Hori M, Nagano H, Wakasa K, Hayashi N, Murakami T.
  • Journal Title: European Journal of Radiology Volume: 20 Pages: 2405-2413
  • Peer Reviewed
• [Journal Article] Comparison of enhancement patterns of histologically confirmed hepatocellular carcinoma between gadoxetate- and ferucarbotran-enhanced magnetic resonance imaging (2010)
  • Author(s): Okada M, Imai Y, Kim T, Kogita S, Takamura M, Kumano S, Onishi H, Hori M, Fukuda K, Hayashi N, Wakasa K, Sakamoto M, Murakami T.
  • Journal Title: Journal of Magnetic Resonance Imaging Volume: 32 Pages: 903-913
  • Peer Reviewed
• [Presentation] Can Human Organic Anion Transporting Polypeptide Mediate the Uptake of Gadoxetate Disodium into Hepatocytes?、Radiological Society of North America(RSNA) (2011)
  • Author(s): Onishi H, Kim T, Hori M, Tatsumi M, Nakamoto A, Tsuboyama T, Tomiyama N
  • Organizer: 97th Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-11-30
• [Presentation] Can Human Organic Anion Transporting Polypeptide Mediate the Uptake of Gadoxetate Disodium into Hepatocytes? (2011)
  • Author(s): Onishi H, Kim T, Hori M, Tatsumi M, Nakamoto A, Tsuboyama T, Tomiyama N
  • Organizer: Radiological Society of North America (RSNA), 97th Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, U.S.A.
  • Year and Date: 2011-11-30
• [Presentation] Gd-EOB-DTPA造影MRIにおける限局性結節性過形成の画像所見に関する検討 (2011)
  • Author(s): 大西裕満、金東石、堀雅敏、巽光朗、坪山尚寛、中本篤、大須賀慶悟、前田登、東原大樹、富山憲幸
  • Organizer: 第70回日本医学放射線学会総会
  • Place of Presentation: (WEB開催)
• [Presentation] ランチョンセミナーV肝臓MRI : CO-OPERATIONの功績肝細胞特異性MR造影剤EOB・プリモビストと薬物トランスポーター～造影剤の肝細胞取り込み機序について～ (2010)
  • Author(s): 大西裕満
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2010-10-01
• [Presentation] ランチョンセミナーV 肝臓MRI:CO-OPERATIONの功績 肝細胞特異性MR造影剤EOB・プリモビストと薬物トランスポーター～造影剤の肝細胞取り込み機序について～ (2010)
  • Author(s): 大西裕満
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2010-10-01
• [Presentation] Gd-EOB-DTPAと薬物トランスポーター～MRIによる生体分子イメージングの可能性～ (2010)
  • Author(s): 大西裕満
  • Organizer: 大阪大学大学院医学系研究科放射線統合医学講座同門会講演会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2010-04-24
• [Presentation] Gd-EOB-DTPAと薬物トランスポーター～MRIによる生体分子イメージングの可能性～ (2010)
  • Author(s): 大西裕満
  • Organizer: 大阪大学大学院医学系研究科 放射線統合医学講座同門会講演会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2010-04-24
• [Presentation] 多血性肝細胞癌におけるGd-EOB-DTPA造影ダイナミックMRIの診断能:造影ダイナミックCTとの比較 (2010)
  • Author(s): 大西裕満、村上卓道、今井康陽、金東石、堀雅敏、坪山尚寛、中本篤、中矢泰裕、高村学、熊野正士
  • Organizer: 第69回日本医学放射線学会学術集会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-04-10
• [Presentation] Gd-EOB-DTPAの肝細胞取り込みに関与するトランスポーターの同定:遺伝子導入卵母細胞を用いた検討 (2010)
  • Author(s): 大西裕満、金東石、堀雅敏、巽光朗、坪山尚寛、中本篤、大須賀慶悟、前田登、東原大樹、友田要
  • Organizer: 第69回日本医学放射線学会学術集会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-04-10
• [Presentation] 肝細胞癌のGd-EOB-DTPA造影肝細胞相における造影効果とトランスポーター発現および胆汁貯留の関連 (2010)
  • Author(s): 坪山尚寛、大西裕満、金東石、秋田裕史、堀雅敏、巽光朗、中本篤、永野浩昭、松浦成昭、若狭研一
  • Organizer: 第69回日本医学放射線学会学術集会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-04-10
• [Presentation] Hypervascular Hepatocellular Carcinomas : Detection with Gd-EOB-DTPA-enhanced MR Imaging and Contrast-enhanced Dynamic MDCT、Radiological Society of North America(RSNA) (2009)
  • Author(s): Onishi H, Murakami T, Kim T, Hori M, Imai Y, Nagano H, Tsuboyama T, Nakaya Y, Nakamoto A, Kumano S, Takamura M
  • Organizer: 95th Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-12-01
• [Presentation] MR Cholangiography with Gd-EOB-DTPA : Biliary Enhancement Dynamics in Clinical Patients (2009)
  • Author(s): Onishi H, Kim T, Hori M, Murakami T, Tatsumi M, Nakaya Y, Nakamoto A, Tsuboyama T, Osuga K, Higashihara H, Maeda N, Tomoda K, Nakamura H
  • Organizer: International Society for Magnetic Resonance in Medicine (17^<th> Scientific Meeting & Exhibition)
  • Place of Presentation: Honolulu, U.S.A.
• [Presentation] Kinetic Analysis of Gd-EOB Uptake by Hepatocytes with Gd-EOB-enhanced Dynamic MR imaging (2009)
  • Author(s): Onishi H, Kim T, Hori M, Murakami T, Tatsumi M, Nakaya Y, Nakamoto A, Tsuboyama T, Osuga K, Higashihara H, Maeda N, Tomoda K, Nakamura H
  • Organizer: International Society for Magnetic Resonance in Medicine (17^<th> Scientific Meeting & Exhibition)
  • Place of Presentation: Honolulu, U.S.A.
• [Presentation] Hypervascular Hepatocellular Carcinomas : Detection with Gd-EOB-DTPA-enhanced MR Imaging and Contrast-enhanced Dynamic MDCT (2009)
  • Author(s): Onishi H, Murakami T, Kim T, Hori M, Imai Y, Nagano H, Tsuboyama T, Nakaya Y, Nakamoto A, Kumano S, Takamura M
  • Organizer: 95^<th> Radiological Society of North America
  • Place of Presentation: Chicago, U.S.A.
• [Book] 画像診断別冊KEY BOOKシリーズ肝胆膵の画像診断. CT・MRIを中心に (2010)
  • Author(s): 大西裕満, 他
  • Publisher: 秀潤社
• [Book] 画像診断別冊KEY BOOKシリーズ肝胆膵の画像診断-CT・MRIを中心に- (2010)
  • Author(s): 大西裕満, 他
  • Publisher: 秀潤社
• [Book] Diagnosis of Hepatocellular Carcinoma : Multidetector-Row Computed Tomography and Magnetic Resonance Imaging (2009)
  • Author(s): Onishi H, Murakami T, Nakamura H
  • Publisher: Springer
• [Book] Diagnosis of Hepatocellular Carcinoma : Multidetector-Row Computed Tomography and Magnetic Resonance Imaging. In : Hayat MA, ed.Methods of Cancer Diagnosis, Therapy, and Prognosis. Volume 5 Liver Cancer (2009)
  • Author(s): Onishi H, Murakami T, Nakamura H
  • Total Pages: 15
  • Publisher: Springer