| Project/Area Number |
21791259
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KATSUDA Masahiro Wakayama Medical University, 医学部, 学内助教 (50464673)
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|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | TLR-9 / CpG-ODN / 癌ワクチン / 食道癌 |
|---|
| Research Abstract |
In inducing Flu peptide-specific CTLs in vitro, Flu peptide-specific cytotoxicity was enhanced in the presence of each type of CpG-ODN and especially CpG-A and CpG-C showed higher cytotoxic activity than CpG-B on PDC dependent manner. URLC10 peptide specific cytotoxicity was enhanced in the presence of CpG-A on PDC dependent manner. In vitro stimulation with CpG-ODN increased the precursor frequency of peptide-specific CTLs within PBMCs derived from esophageal cancer patients. The supernatant derived from PBMCs stimulated with CpG-ODN (CpG-sup) and IFN-α augmented the cytotoxicity of URLC10 specific CTL clone. These results revealed type-1 IFN induced by CpG-ODNs augmented the cytotoxic activity of the peptide-specific CTLs, cleary revealing one of the mechanism how CpG-ODNs would work as an ideal adjuvant for peptide vaccine therapy against cancer.
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