Approaches to regenerate pancreatic function by converting liver blood flow and transferring specific gene

• Project/Area Number: 21791282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Shiga University of Medical Science
• Principal Investigator: FUJINO Kazunori 滋賀医科大学, 医学部, 助教 (70402716)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵再生 / 遺伝子治療 / ファージベクター

Research Abstract

Although we found the both anti-insulin and anti-trypsin positive cells in the liver that interrupted portal blood flow by the immunohistochemical study, that immature cell cluster didn' t have any secretary granules. In this study, we attempted to introduce differentiation enhancing factor to the cluster by gene therapy, and generate pancreatic endocrine and exocrine cells. Although we try to make phage vector to deliver differentiation enhancing factor to the cluster for these research period, we couldn' t find adequate amount vector-transfected cells in both the liver and bile duct. We will continue to make vector for it in the future.

Research Products

• [Journal Article] Homing of the bone marrow-derived interstitial cells of Cajal is decreased in diabetic mouse intestine (2011)
  • Author(s): Li Y, Kojima H, Fujino K, Matsumura K, Katagi M, Urabe H, Chan L, Eguchi Y, Zhao L, Kimura H.
  • Journal Title: J Gastroenterol Hepatol Volume: Vol.26, no.6 Pages: 1072-1078
  • Peer Reviewed
• [Journal Article] Ghrelin family of peptides and gut motility (2011)
  • Author(s): Asakawa A, Ataka K, Fujino K, Chen CY, Kato I, Fujimiya M, Inui A.
  • Journal Title: J Gastroenterol Hepatol, suppl Volume: no.3 Pages: 73-74
  • Peer Reviewed
• [Journal Article] Homing of the bone marrow-derived interstitial cells of Cajal is decreased in diabetic mouse intestine (2011)
  • Author(s): Li Y
  • Journal Title: J Gastroenterol Hepatol Volume: 26 Pages: 1072-1078
  • Peer Reviewed
• [Journal Article] Parathyroid hormone-related protein has an anorexigenic activity via activation of hypothalamic urocortins 2 and 3 (2010)
  • Author(s): Asakawa A, Fujimiya M, Niijima A, Fujino K, Kodama N, Sato Y, Kato I, Nanba H, Laviano A, Meguid MM, Inui A.
  • Journal Title: Psychoneuroendocrinology Volume: Vol.35, No.8 Pages: 1178-1186
  • Peer Reviewed