Establishment and characterization of cancer stem cell model using iPS technology

• Project/Area Number: 21791307
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Keio University
• Principal Investigator: FUKUDA Kazumasa Keio University, 医学部, 助教 (50348786)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: iPS / 人工癌幹細胞 / 胃癌 / リプログラミング / 癌幹細胞

Research Abstract

We demonstrate induction of iCaPS from human gastric cancer (GC) cell lines and primary culture cell derived from the patients with GC introducing Yamanaka four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. These cells, which we designated iCaPS, exhibit the morphology and growth properties of iPS cells. These data suggest that CSCs (cancer stem-like cells) can be directly generated from human GC cell liens by the addition of only a few defined factors. We analysed expression of alkaline phosphatase. These date indicated that iCaPS cells acquired immature status. WST-8 assay showed that iCaPS cells were acqired low sensitivityto fluorouracil, cisplatin and PTX compred with wild type cell. As novel therapeutic approches in GC, the properties of iCaPS should bebe investigaged.