In situ analysis of crossbridge dynamics in failing heart model of rat using X-ray technique

• Project/Area Number: 21791330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Nara Medical University
• Principal Investigator: TAMURA Yamato Nara Medical University, 医学部, 研究員 (20382301)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 化学物理 / 加速器 / 心不全 / X線解析

Research Abstract

This study was aimed for the development of the method to give heart failure a diagnosis by observing dynamics of the molecule nano-order using SPring-8. We paid attention to particularly myocardial contractile protein and analyzed actin-myosin interaction and lattice spacing between myosin filaments used the rats which maintained complicated form and a multiplex compensation mechanism and the heart failure model.
We performed the measurement of the hypertrophic heart model by isoproterenol, analyzed crossbridge dynamics in the diastolic phase and the contraction phase and of the hypertrophic heart and evaluated the failing heart.

Research Products

• [Presentation] Increasing preload reduced actin-myosin interaction beating rat whole heart (2010)
  • Author(s): Yamto Tamura, Takehiro Miyasaka, Juichiro Shimizu
  • Organizer: Cardiovascular System Dynamics Society
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2010-09-26
• [Presentation] 低酸素環境下での前負荷増大による心機能低下と心筋分子モータの挙動異常 (2010)
  • Author(s): 清水壽一郎、宮坂武寛、田村大和
  • Organizer: 第25回生体・生理工学シンポジウム
  • Place of Presentation: 岡山大学鹿田キャンパス 岡山
  • Year and Date: 2010-09-25
• [Presentation] 低酸素環境下での前負荷増大による心機能低下と心筋分子モータ挙動異常 (2010)
  • Author(s): 田村大和
  • Organizer: 第25回生体・生理工学シンポジウム
  • Place of Presentation: 岡山大学大学院保健学研究科
  • Year and Date: 2010-09-25
• [Presentation] 低酸素灌流時の前負荷の増大はラット摘出心標本における心筋アクチンミオシン相互作用を減少させる (2009)
  • Author(s): 田村大和、清水壽一郎、宮坂武寛、竹下大輔、吉川義朗、谷口繁樹
  • Organizer: 第62回日本胸部外科学会定期学術集会
  • Place of Presentation: パシフィコ横浜 横浜
  • Year and Date: 2009-10-14
• [Presentation] 低酸素灌流時の前負荷の増大はラット摘出心標本における心筋アクチンミオシン相互作用を減少させる (2009)
  • Author(s): 田村大和
  • Organizer: 第62回日本胸部外科学会総会
  • Place of Presentation: 横浜パシフィコ横浜
  • Year and Date: 2009-10-14
• [Presentation] Increasing preload reduced actin-myosin interaction in isolated beating rat whole heart under hypoxia (2009)
  • Author(s): Yamato Tamura, Juichiro Shimizu, Takehiro Miyasaka, Daisuke Takeshita, Naoto Yagi
  • Organizer: Medical Physics and Biomedical Engineering
  • Place of Presentation: Munich, Germany
  • Year and Date: 2009-09-09
• [Presentation] Increasing Preload Reduced Actin-Myosin Interaction in Isolated Beating Rat Whole Heart Under Hypoxia (2009)
  • Author(s): Yamato Tamura
  • Organizer: Medical physics and biomedical engineering World congress 2009
  • Place of Presentation: Munich, Germany
  • Year and Date: 2009-09-09
• [Presentation] Actin-myosin interaction observed in beating whole heart under physiological and pathological states (2009)
  • Author(s): Juichiro Shimizu, Yamato Tamura, Takehiro Miyasaka
  • Organizer: Third Switzerland-Japan Workshop on biomechanics
  • Place of Presentation: Engelberg, Switzerland
  • Year and Date: 2009-09-04
• [Presentation] Increasing heart rate decreased actin-myosin interaction in isolated beating rat whole heart (2009)
  • Author(s): Juichiro Shimizu, Daisuke Takeshita, Yamato Tamura, Tatsuhito Matsuo, Takehiro Miyasaka, Miyako Takaki, Naoto Yagi
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-08-01
• [Presentation] 心拍数の増加は拡張期の残存クロスブリッジを増加させる (2009)
  • Author(s): 田村大和、清水壽一郎、竹下大輔、宮坂武寛、八木直人
  • Organizer: 第30回日本循環制御医学会総会
  • Place of Presentation: 鹿児島市民文化ホール、鹿児島
  • Year and Date: 2009-06-12
• [Presentation] 心拍数の増加はアクチン-ミオシンの架橋形成を減少させる (2009)
  • Author(s): 宮坂武寛、清水壽一郎、田村大和、竹下大輔、高木都、八木直人
  • Organizer: 第48回日本生体医工学会大会
  • Place of Presentation: タワーホテル船堀、東京
  • Year and Date: 2009-04-25