| Project/Area Number |
21791338
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KANEDA Hiroyuki Kansai Medical University, 医学部, 助教 (20411522)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 癌幹細胞 / ニッチ / 肺癌 / 薬剤抵抗性 / 免疫染色 |
|---|
| Research Abstract |
In 2009, immunohisto-chemical examination to identify lung cancer stem cells using CD133 antibody showed that CD133 positive cells in lung cancer were very rare. However, flow cytometry examination revealed that percentage of sorted CD133 positive cells was 9.1% on average. In 2010, examination with sorted side population cells did not work well because of low rate of sorted cells. To examine our hypothesis that location of carcinogenesis in whole lung has bias, we reviewed clinical characteristics and site of lobe of the tumor in lung cancer patients who had multiple tumors. Right upper lobe had significantly more tumors than other lobes in multiple lung cancer patients, suggesting that something related with carcinogenesis highly occur more in right upper lobe. Gene mutation analysis of those patients with multiple tumors suggested some specific gene might be related with those sort of the carcinogenesis.
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