Assessment of prion inactivation with quantitative Protein Misfolding Cyclic Amplification

• Project/Area Number: 21791343
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Tohoku University
• Principal Investigator: TAKEUCHI Atsuko Tohoku University, 大学院・医学系研究科, 助教 (00535239)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: プリオン病 / 脳神経疾患 / 蛋白質 / 臨床 / 衛生

Research Abstract

To evaluate prion removal efficacy of many disinfectants, multi-round PMCA was applied. We then analysed several sets of serial 10-fold dilutions of mock-treated samples statistically, and found that PMCA round numbers linearly correlate with means of dilution factors of infected brain between the second and sixth round. We found that a combined use of alkaline cleaner and autoclaving (121℃, 15min) was one of the most effective treatments for prion inactivation, and the log reduction factor of PrP^<res> was estimated to be ≧7log.

Research Products

• [Journal Article] Heparin enhances the cell-protein misfolding cyclic amplification efficiency of variant Creutzfeldt-Jakob disease. (2011)
  • Author(s): Yokoyama T, Takeuchi A, Yamamoto M, Kitamoto T, Ironside JW, Morita M.
  • Journal Title: Neuroscience Letters. in press
  • Peer Reviewed
• [Journal Article] Deduction of the evaluation limit and termination timing of multi-round protein misfolding cyclic amplification from a titration curve. (2011)
  • Author(s): Takeuchi A, Komiya M, Kitamoto T, Morita M.
  • Journal Title: Microbiology and Immunology in press
  • NAID: 10031121496
  • Peer Reviewed
• [Journal Article] Deduction of the evaluation limit and termination timing of multi-round protein misfolding cyclic amplification from a titration curve (2011)
  • Author(s): Atsuko Takeuchi, Mayumi Komiya, Tetsuyuki Kitamoto, Masanori Morita
  • Journal Title: Microbiology and Immunology Volume: (未定 印刷中)
  • NAID: 10031121496
  • Peer Reviewed
• [Presentation] リコンビナントPrP^cを用いたPMCA法によるヒトプリオンの増幅 (2009)
  • Author(s): 竹内敦子, 山本幸、北本哲之、森田将典
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Remarks] ホームページ
  • URL: http://www.prion.med.tohoku.ac.jp/