Assessment of prion inactivation with quantitative Protein Misfolding Cyclic Amplification
Project/Area Number |
21791343
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Cerebral neurosurgery
|
Research Institution | Tohoku University |
Principal Investigator |
TAKEUCHI Atsuko Tohoku University, 大学院・医学系研究科, 助教 (00535239)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | プリオン病 / 脳神経疾患 / 蛋白質 / 臨床 / 衛生 |
Research Abstract |
To evaluate prion removal efficacy of many disinfectants, multi-round PMCA was applied. We then analysed several sets of serial 10-fold dilutions of mock-treated samples statistically, and found that PMCA round numbers linearly correlate with means of dilution factors of infected brain between the second and sixth round. We found that a combined use of alkaline cleaner and autoclaving (121℃, 15min) was one of the most effective treatments for prion inactivation, and the log reduction factor of PrP^<res> was estimated to be ≧7log.
|
Report
(3 results)
Research Products
(5 results)