Co-transplantation of ES Cells and Bone Marrow Stromal Cells for Spinal Cord Injury

• Project/Area Number: 21791375
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: MATSUDA Ryosuke Nara Medical University, 医学部, 助教 (60453164)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脊髄損傷 / 骨髄間質細胞 / ES細胞 / 共培養 / 共移植 / 脊髄間質細胞

Research Abstract

We performed co-transplantation of neural stem cells derived from ES cells and bone marrow stromal cells for spinal cord injury in mice. We checked motor function after spinal cord injury weekly and histopathological examination using neuronal markers. Histopathological examination revealed that neural stem cells derived Es cells differentiated into Nestin positive cells and MAP2 positive cells. There is no significance for motor functional improvement between co-transplantation group and other control groups. The reason for ineffectiveness of this study is unclear. Further study will be required.

Research Products

• [Journal Article] Modulated differentiation of embryonic stem cells into hepatocyte-like cells by coculture with hepatic stellate cells. (2011)
  • Author(s): Nishiofuku M, Yoshikawa M, Ouji Y, Saito K, Moriya K, Ishizaka S, Nishimura F, Matsuda R, Yamada S, Fukui H
  • Journal Title: J Biosci Bioeng 111 Pages: 71-77
  • NAID: 110008440353
  • Peer Reviewed
• [Journal Article] Modulated differentiation of embryonic stem cells into hepatocyte-like cells. by coculture with hepatic stellate cells. (2011)
  • Author(s): Nishiofuku M, Matsuda R, et al
  • Journal Title: J Biosci Bioeng Volume: 111 Pages: 71-77
  • NAID: 110008440353
  • Peer Reviewed
• [Journal Article] Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart. (2010)
  • Author(s): Ouji Y, Yoshikawa M, Moriya K, Nishiofuku M, Ouji-Sageshima N, Matsuda R, Nishimura F, Ishizaka S
  • Journal Title: J Biosci Bioeng 110 Pages: 487-490
  • NAID: 110007880149
  • Peer Reviewed
• [Journal Article] Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart. (2010)
  • Author(s): Ouji Y, Matsuda R, et al
  • Journal Title: J Biosci Bioeng Volume: 110 Pages: 487-490
  • NAID: 110007880149
  • Peer Reviewed
• [Journal Article] Cotransplantation of mouse embryonic Stem cells and bone marrow stromal cells followingspinal cord injury suppresses (2009)
  • Author(s): Matsuda R, Yoshikawa M, Kimura H, Ouji Y, Nakase H, Nishimura F, Nonaka J, Toriumi H, Yamada S, Nishiofuku M, Moriva K, Ishizaka S, Nakamura M, Sakaki T
  • Journal Title: Cell transplantation 18 Pages: 39-54
  • Peer Reviewed
• [Journal Article] Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells : necessity of immunosuppressive treatment for sustained improvement. (2009)
  • Author(s): Toriumi H, Yoshikawa M, Matsuda R, Nishimura F, Yamada S, Hirabayashi Nakase H, Nonaka J, Ouji Y, Ishizaka S, Sakaki T.
  • Journal Title: Neurological Reseach 31 Pages: 220-227
  • Peer Reviewed
• [Journal Article] Co-transplantation of Mouse Embryonic Stem Cells and Bone Marrow Stromal Cells Following Spinal Cord In jury Suppresses Tumor Development (2009)
  • Author(s): Matsuda R, et al
  • Journal Title: Cell Transplantation Vol 18 Pages: 39-54
  • Peer Reviewed
• [Journal Article] Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells : necessity of immunosuppressive treatment for sustained improvement. (2009)
  • Author(s): Toriumi H, Matsuda R, et al.
  • Journal Title: Neurological Reaseach Vol 31(3) Pages: 220-227
  • Peer Reviewed
• [Presentation] Stress Induced Prematured Senescence in Glioblastoma Cell Line and Relationship with Carnitine (2010)
  • Author(s): 山田修一, 松田良介, 西村文彦, 朴永銖, 中村光利, 中瀬裕之, 王寺幸輝, 吉川正英, 石坂重明
  • Organizer: 日本脳腫瘍学会
  • Place of Presentation: 軽井沢
  • Year and Date: 2010-11-28
• [Presentation] Stress Induced Prematured Senescence in Glioblastoma Cell Line and Relationship with Carnitine (2010)
  • Author(s): 山田修一, 松田良介, 西村文彦, 朴永銖, 中村光利, 中瀬裕之, 王寺幸輝, 吉川正英, 石坂重明
  • Organizer: 日本分子脳神経外科学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-08-28
• [Presentation] 脊髄損傷に対するES細胞移植治療の問題点 (2009)
  • Author(s): 松田良介, 山田修一, 中瀬裕之, 王寺幸輝, 吉川正英, 石坂重明
  • Organizer: 日本分子脳神経外科学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-09-20
• [Remarks] ホームページ等