Identification of novel growth factor for Glioma cancer stem cells

• Project/Area Number: 21791377
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Keio University
• Principal Investigator: TAKAHASHI Satoshi Keio University, 医学部, 研究員 (20383870)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 脳腫瘍学 / 腫瘍幹細胞 / 脳腫瘍 / 幹細胞 / 増殖因子 / 神経膠腫 / 脳腫瘍幹細胞

Research Abstract

We have conducted our research for the purpose of identifying novel growth factors for glioma cancer stem cells, and identified KIF23 and uPARAP. Downregulation of KIF23 in glioma cells suppressed their proliferation. Downregulation of uPARAP in glioma cells suppressed migration and invasion properties of glioma cells through changes in actin cytoskeleton. We have also confirmed the expressions of the both molecules in glioma cancer stem cells at protein level.

Research Products

• [Journal Article] Downregulation of uPARAP mediates cytoskeletal rearrangements and decreases invasion and migration properties in glioma cells. (2011)
  • Author(s): Takahashi S, Yamada-Okabe H, Hamada K, et al.
  • Journal Title: J Neurooncol. 103(2) Pages: 267-276
  • Peer Reviewed
• [Journal Article] Downregulation of uPARAP mediates cytoskeletal rearrangements and decreases invasion and migration properties in glioma cells. (2010)
  • Author(s): Takahashi S, Yamada-Okabe H, Hamada K, Ohta S, Kawase T, Yoshida K, Toda M
  • Journal Title: J Neurooncol Volume: (in press)
  • Peer Reviewed
• [Presentation] 脳腫瘍幹細胞・グリオーマ共通抗原の発現および機能解析 (2010)
  • Author(s): 戸田正博、田伏将尚、大多茂樹、深谷雷太、高橋里史、吉田一成
  • Organizer: 第28回日本脳腫瘍学会学術集会
  • Place of Presentation: 軽井沢
  • Year and Date: 2010-11-29
• [Presentation] GliomaにおけるKIF23発現の検討と機能解析 (2010)
  • Author(s): 高橋里史, 戸田正博, 吉田一成
  • Organizer: 第69回社団法人日本脳神経外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-28
• [Presentation] GliomaにおけるKIF23発現の検討と機能解析 (2010)
  • Author(s): 高橋里史、戸田正博、吉田一成
  • Organizer: 日本脳神経外科学会第69回学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-28
• [Presentation] RNA interference-mediated down-regulation of uPARAP decreases invasion and migration property in glioma cells through reorganization of the actin cytoskeleton. (2010)
  • Author(s): Takahashi S, Toda M, Yamada-Okabe H, Hamada K, Kawase T, Yoshida K
  • Organizer: 慶應ニューロサイエンス研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-29
• [Presentation] RNA interference-mediated down-regulation of uPARAP decreases invasion and migration property in glioma cells through reorganization of the actin cytoskeleton. (2010)
  • Author(s): Takahashi S, Toda M, Yamada-Okabe H, Hamada K, Kawase T, Yoshida K
  • Organizer: 慶應ニューロサイエンス
  • Place of Presentation: 東京
  • Year and Date: 2010-05-29
• [Presentation] RNA interference-mediated downregulation of uPARAP decreases invasion and migration property in glioma cells through reorganization of the actin cytoskeleton (2010)
  • Author(s): Satoshi Takahashi, Masahiro Toda, Hikaru Sasaki, Takeshi Kawase.
  • Organizer: AACR 101^<st> annual meeting 2010
  • Place of Presentation: Washington, D.C.
  • Year and Date: 2010-04-18
• [Presentation] siRNAを用いたuPARAP knock downはGlioma培養細胞の移動・浸潤能を抑制する (2010)
  • Author(s): 高橋里史, 河瀬斌, 戸田正博
  • Organizer: 第5回「脳腫瘍の基礎シンポジウム」
  • Place of Presentation: 東京
  • Year and Date: 2010-01-30
• [Presentation] P.002 Down-regulation of membrane protein uPARAP makes glioma cells immobile and can be a target for novel glioma therapy (2010)
  • Author(s): S.Takahashi, T.Kawase, M.Toda
  • Organizer: 9^<th> congress of the European association of neurooncology
  • Place of Presentation: Maastricht, Netherland
• [Presentation] Down-regulation of membrane protein uPARAP makes glioma cells immobile and can be a target for novel glioma therapy (2010)
  • Author(s): Takahashi S, Kawase T, Toda M
  • Organizer: 9^<th> congress of the European association of neurooncology
  • Place of Presentation: Maastricht
• [Presentation] GliomaにおけるuPARAP発現の検討と機能解析 (2009)
  • Author(s): 高橋里史, 戸田正博, 河瀬斌
  • Organizer: 第68回社団法人日本脳神経外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-16