Musashi1 modulates glioma cell growth caused due to the regulation of cell cycle
| Project/Area Number |
21791378
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Keio University |
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Principal Investigator |
MUTOH Jun Keio University, 医学部, 助教 (30383839)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 癌 / 遺伝子 / 脳疾患 / 悪性神経膠芽腫 / 腫瘍幹細胞 / Musashi1 / 細胞増殖 / 脳腫瘍 / 癌幹細胞 / Musashil |
|---|
| Research Abstract |
The expression of Musashi1 (MSI1), an RNA-binding protein that plays a crucial role in nervous system development and stem cell self-renewal. Here, we examined its role in the progression of glioma. The RNAi-based MSI1-knock down (KD) in glioblastoma and medulloblastoma cells resulted in a significantly lower number of colony forming cells on day 30 (66% reduction in colony size), indicative of the inhibitory effect of MSI1-KD on tumor cell growth. Immunocytochemical staining of MSI1-KD glioblastoma cells suggested that these cells ectopically express markers of Meta-phase. A 2.2 -fold increased number of MSI1-KD cells in the G2/M phase, as detected by fluorescence cell sorting, further confirmed the induction of mitosis and defect of cell survival upon MSI1-KD treatment, although it did not alter the expression of, activated caspase-3. We further showed that the xenografts of MSI1-KD glioblastoma cells had a 96.6% reduction of tumor size measured by bioluminescence imaging system(BLI) and longer survival (49.3±6.1 days) than the control group (33.6±3.6 days ; P<0.01). Taken together, these findings and gene analysis suggest that in MSI1-KD in glioma cells results in the prolongation of the cell cycle by accumulating cyclin B and reduced activity of Notch signal pathway accompanying with up-regulation of m-Numb, which could cause the decrease of survival of glioma cells..
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Report
(3 results)
Research Products
(26 results)
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[Presentation] Development and analysis of transient middle cerebral artery occlusion model on Common marmoset2011
Author(s)
Hara K, Muto J, Inoue S, Kaneko N, Adachi K, Toyota F, Hikishima S, Ito T, Sawamoto K, Kawase T, Yoshida K, Okano H.
Organizer
The 10^<th> congress of the Japanese society for regenerative medicine.
Place of Presentation
(Tokyo, JAPAN)(Japanese)
Year and Date
2011-03-02
Related Report
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[Presentation] Development and analysis of transient middle cerebral artery occlusion model on Common marmoset2010
Author(s)
Hara K, Muto J, Inoue S, Kaneko N, Adachi K, Toyota F, Hikishima S, Ito T, Sawamoto K, Kawase T, Yoshida K, Okano H.
Organizer
68^<th> The Japanese Society for Neurosurgery.
Place of Presentation
Japanese
Year and Date
2010-10-29
Related Report
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[Presentation] Development and analysis of transient middle cerebral artery occlusion model on Common marmoset2010
Author(s)
Hara K, Muto J, Inoue S, Kaneko N, Adachi K, Toyota F, Hikishima S, Ito T, Sawamoto K, Kawase T, Yoshida K, Okano H.
Organizer
The 10^<th> congress of the Japanese Society for Regeneration
Place of Presentation
(Hiroshima JAPAN)(Japanese)
Year and Date
2010-03-11
Related Report
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[Presentation] Development and analysis of transient middle cerebral artery occlusion model on Common marmoset2009
Author(s)
Hara K, Muto J, Inoue S, Kaneko N, Adachi K, Toyota F, Hikishima S, Ito T, Sawamoto K, Kawase T, Yoshida K, Okano H.
Organizer
The 10^<th> Annual meeting of the Japan Society of Molecular Neurosurgery
Place of Presentation
(Tokyo JAPAN)(English)
Year and Date
2009-09-19
Related Report
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[Presentation] Skull base epidermoid2009
Author(s)
Muto J, Kawase T, Ohira T, Yoshida K
Organizer
1st Swiss-Japanese neurosurgical conference
Place of Presentation
(Zurich Swiss)(English)
Year and Date
2009-07-23
Related Report
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