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n vivo imaging of bone and soft tissue tumor using fluorescent antibody

Research Project

Project/Area Number 21791387
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

HAYASHI Katsuhiro  Kanazawa University, 附属病院, 助教 (80507054)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords骨・軟部腫瘍学 / 蛍光イメージング / 癌 / 外科
Research Abstract

We have investigated in vivo imaging of bone and soft tissue tumor using fluorescent antibody. Osteosarcoma cells were stained with fluorescent anti-ALP antibody. For distinction of normal tissue, red or near-infrared wavelength was suitable. Osteosarcoma cells were transplanted into mice and ICG was intravenously administered. Tumor was visualized by ICG. It can be used for tumor resection guide. Further investigation would be considered, such as improvement of observation devices.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (29 results)

All 2011 2010 2009

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (14 results)

  • [Journal Article] 転移リアルタイムイメージング2010

    • Author(s)
      林克洋
    • Journal Title

      臨床整外 45

      Pages: 1134-1138

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium.2010

    • Author(s)
      Kimura H, Zhang L, Zhao M, K Hayashi, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
    • Journal Title

      Cell Prolif 43

      Pages: 41-48

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo.2010

    • Author(s)
      Kimura H, Lee C,K Hayashi, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Hoffman RM.
    • Journal Title

      J Cell Biochem 110

      Pages: 1439-1446

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of single cancer-cell dynamics of lung metastasis.2010

    • Author(s)
      Kimura H,K Hayashi, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM.
    • Journal Title

      J Cell Biochem 109

      Pages: 58-64

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model.2010

    • Author(s)
      Hasegawa A,K Hayashi, Kishimoto H, Yang M, Tofukuji S, Suzuki K, Nakajima H, Hoffman RM, Shirai M, Nakayama T.
    • Journal Title

      J Allergy Clin Immunol 125

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model.2010

    • Author(s)
      Hasegawa A, Hayashi K, et al.
    • Journal Title

      J Allergy Clin Immunol.

      Volume: 125 Pages: 461-468

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of single cancer-cell dynamics of lung metastasis2010

    • Author(s)
      Kimura H, Hayashi K, et al
    • Journal Title

      J Cell Biochem

      Volume: 109 Pages: 58-64

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo2010

    • Author(s)
      Kimura H, Lee C, Hayashi K, et al
    • Journal Title

      J Cell Biochem

      Volume: 110 Pages: 1439-46

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 転移リアルタイムイメージング2010

    • Author(s)
      林克洋
    • Journal Title

      臨床整外

      Volume: 45 Pages: 1134-1138

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis.2009

    • Author(s)
      Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Wessels J, Hoffman RM.
    • Journal Title

      Cell Prolif 42

      Pages: 75-82

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhimurium.2009

    • Author(s)
      Hayashi K, Zhao M, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Hoffman RM.
    • Journal Title

      J Cell Biochem 106

      Pages: 992-998

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential.2009

    • Author(s)
      Tome Y, Tsuchiya H,K Hayashi, Yamauchi K, Sugimoto N, Kanaya F, Tomita K, Hoffman RM.
    • Journal Title

      J Cell Biochem 108

      Pages: 362-367

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhimurium.2009

    • Author(s)
      Hayashi K, et al
    • Journal Title

      Cell Cycle 8

      Pages: 870-875

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhimurium.2009

    • Author(s)
      Hayashi K, et al
    • Journal Title

      J Cell Biochem 106

      Pages: 992-998

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis.2009

    • Author(s)
      Hayashi K, et al
    • Journal Title

      Cell Prolif. 42

      Pages: 75-82

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Leading technology for in vivo fluorescent sarcoma imaging2011

    • Author(s)
      Hayashi K, et al
    • Organizer
      American Academy of Orthopaedic Surgeons
    • Place of Presentation
      San Diego Convention Center (USA)
    • Year and Date
      2011-02-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Leading technology for in vivo fluorescent sarcoma imaging.2011

    • Author(s)
      Katsuhiro Hayashi, Norio Yamamoto, Toshiharu Shirai, Hideji Nishida, Akihiko Takeuchi, Hiroaki Kimura, Robert M Hoffman, Hiroyuki Tsuchiya.
    • Organizer
      AAOS American Academy of Orthopaedic Surgeons
    • Place of Presentation
      San Diego Convention Center (USA)
    • Related Report
      2010 Final Research Report
  • [Presentation] サルモネラ変異株を応用したリンパ節転移の治療と蛍光イメージング2010

    • Author(s)
      林克洋、土屋弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2010-10-14
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Targeting of primary tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium.2010

    • Author(s)
      Hayashi K, Tsuchiya H.
    • Organizer
      日本整形外科学会学術総会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2010-05-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Targeting of primary tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium2010

    • Author(s)
      Hayashi K, et al
    • Organizer
      日本整形外科学会学術総会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2010-05-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング2009

    • Author(s)
      林克洋, 土屋弘行, 山本憲男, 白井寿治, 山内健輔, ロバートホフマン, 富田勝郎
    • Organizer
      第18回日本がん転移学会学術集会・総会
    • Place of Presentation
      旭川グランドホテル(北海道)
    • Related Report
      2010 Final Research Report
  • [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング2009

    • Author(s)
      林克洋、土屋弘行
    • Organizer
      第42回日本整形外科学会骨軟部腫瘍学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2010 Final Research Report
  • [Presentation] Dual color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma.2009

    • Author(s)
      林克洋、土屋弘行
    • Organizer
      第52回日本整形外科学会学術総会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2010 Final Research Report
  • [Presentation] Real Time Imaging of Cancer Cell Seeding to the Lung in Live Mouse.2009

    • Author(s)
      K Hayashi, H Tsuchiya, N Yamamoto, T Shirai, H Nishida, K Yamauchi, A Takeuchi, H Kimura, K Tomita, M Bouvet, RM.Hoffman.
    • Organizer
      100th American Association for Cancer Research
    • Place of Presentation
      Colorado Convention Center (USA)
    • Related Report
      2010 Final Research Report
  • [Presentation] Real Time Imaging of Cancer Cell Seeding to the Lung in Live Mouse2009

    • Author(s)
      林克洋
    • Organizer
      100th American Association for Cancer Research
    • Place of Presentation
      Colorado Convention Center(デンバー(米国))
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dual color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma.2009

    • Author(s)
      林克洋
    • Organizer
      第52回日本整形外科学会学術総会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング2009

    • Author(s)
      林克洋
    • Organizer
      第42回日本整形外科学会骨軟部腫瘍学術集会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛍光蛋白を使用した癌細胞のリンパ節転移のリアルタイムイメージング2009

    • Author(s)
      林克洋
    • Organizer
      第18回日本がん転移学会学術集会・総会
    • Place of Presentation
      旭川グランドホテル(旭川)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛍光蛋白を使用した悪性腫瘍の転移リアルタイムイメージング2009

    • Author(s)
      林克洋
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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