Regeneration of spinal cord injury using microRNA

• Project/Area Number: 21791400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: NAKANISHI Kazuyoshi Hiroshima University, 病院, 病院助教 (60403557)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脊髄 / microRNA / 組織再生 / micro RNA / micro RMA

Research Abstract

MicroRNA223 shows high levels of expression at 12 hours and 3days after spinal cord injury using PCR in mice. And microRNA-223 and inflammatory cytokines also shows significantly high levels of expression within the range of 2mm of the head and caudal around the epicenter at 12 hours after spinal cord injury by PCR. Immunohistochemistry revealed 60% of the microRNA-223 positive cells merged with the Gr-1 positive neutrophils. Therefore, the accumulation of the neutrophils and inflammatory cytokine occurs within the range of the 2mm of head and caudal around the epicenter. And it was suggested that microRNA-223 is related to the expression of neutrophils in the early response of spinal cord injury.

Research Products

• [Journal Article] MicroRNA-223 expression in neutrophils in the early phase of secondary damage after spinal cord injury. (2011)
  • Author(s): Izumi B, Nakasa T, Tanaka N, Nakanishi K, Kamei N, Yamamoto R, Nakamae T, Ohta R, Fujioka Y, Yamasaki K, Ochi M.
  • Journal Title: Neuroscience Letters 492 Pages: 114-118
  • Peer Reviewed
• [Journal Article] MicroRNA-223 expression in neutrophils in the early phase of secondary damage after spinal cord injury. (2011)
  • Author(s): Izumi B, Nakasa T, Tanaka N, Nakanishi K, Kamei N, Yamamoto R, Nakamae T, Ohta R, Fujioka Y, Yamasaki K, Ochi M.
  • Journal Title: Neuroscience Letters Volume: (In Press)
  • Peer Reviewed
• [Journal Article] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2010)
  • Author(s): 泉文一郎, 中佐智幸, 田中信弘, 中西一義, 亀井直輔, 山本りさこ, 中前稔生, 大田亮, 藤岡悠樹, 越智光夫
  • Journal Title: 脊髄機能診断学 32巻 Pages: 69-72
  • Peer Reviewed
• [Journal Article] Responses of microRNAs 124a and 223 following spinal cord injury in mice. (2010)
  • Author(s): Nakanishi K, Nakasa T, Tanaka N, Ishikawa M, Yamada K, Yamasaki K, Kamei N, Izumi B, Adachi N, Miyaki S, Asahara H, Ochi M
  • Journal Title: Spinal Cord 48(3) Pages: 192-196
  • Peer Reviewed
• [Journal Article] Responses of microRNAs 124a and 223 following spinal cord injury in mice. (2010)
  • Author(s): Nakanishi K, Nakasa T, Tanaka N, Ishikawa M, Yamada K, Yamasaki K, Kamei N, Izumi B, Adachi N, Miyaki S, Asahara H, Ochi M
  • Journal Title: Spinal Cord Volume: 48(3) Pages: 192-196
  • Peer Reviewed
• [Journal Article] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2010)
  • Author(s): 泉文一郎
  • Journal Title: 脊髄機能診断学 Volume: 32巻 Pages: 69-72
  • Peer Reviewed
• [Journal Article] Responses of microRNAs 124a and 223 following spinal cord injury in mice. (2009)
  • Author(s): Nakanishi K
  • Journal Title: Spinal Cord 21 Pages: 192-196
  • Peer Reviewed
• [Presentation] MicroRNA-223 was expressed in the neutrophils at the early period of secondary damage after spinal cord injury Orthopaedic Research Society (2011)
  • Author(s): Izumi B, Nakasa T, Tanaka N, Nakanishi K, Kamei N, Yamamoto R, Nakamae T, Ohta R, Fujioka Y, Ochi M.
  • Organizer: 57^<th> Annual Meeting
  • Place of Presentation: Los Angeles USA.
  • Year and Date: 2011-01-13
• [Presentation] MicroRNA-223 was expressed in the neutrophils at the early period of secondary damage after spinal cord injury (2011)
  • Author(s): 泉文一郎
  • Organizer: Orthopaedic Research Society 57^<th> Annual Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2011-01-13
• [Presentation] MicroRNA-223 was expressed in the neutrophils at the early period of secondary damage after spinal cord injury (2010)
  • Author(s): Izumi B, Nakasa T, Tanaka N, Nakanishi K, Kamei N, Yamamoto R, Nakamae T, Ohta R, Fujioka Y, Ochi M.
  • Organizer: 7^<th> combined meeting Orthopaedic Research Society
  • Place of Presentation: Kyoto
  • Year and Date: 2010-10-16
• [Presentation] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2010)
  • Author(s): 泉文一郎, 中佐智幸, 田中信弘, 中西一義, 亀井直輔, 山本りさこ, 中前稔生, 大田亮, 藤岡悠樹, 越智光夫
  • Organizer: 第25回日本整形外科基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-14
• [Presentation] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2010)
  • Author(s): 泉文一郎
  • Organizer: 第25回日本整形外科基礎学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-14
• [Presentation] Expression of microRNA-223 following spinal cord inj ury in mice. (2010)
  • Author(s): Izumi B, Nakasa T, Tanaka N, Nakanishi K, Kamei N, Yamamoto R, Nakamae T, Ohta R, Fujioka Y, Ochi M.
  • Organizer: Orthopaedic Research Society 56^<th> Annual Meeting
  • Place of Presentation: New Orleans USA
  • Year and Date: 2010-04-06
• [Presentation] Expression of microRNA-223 following spinal cord injury in mice. (2010)
  • Author(s): Bunichiro Izumi
  • Organizer: Orthopaedic Research Society 56^<th> Annual Meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2010-03-09
• [Presentation] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2010)
  • Author(s): 泉文一郎
  • Organizer: 第32回脊髄機能診断研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-06
• [Presentation] マウス脊髄損傷モデルにおけるmicroRNA-223の発現 (2009)
  • Author(s): 泉文一郎, 中佐智幸, 田中信弘, 中西一義, 亀井直輔, 山本りさこ, 中前稔生, 大田亮, 藤岡悠樹, 越智光夫
  • Organizer: 第32回脊髄機能診断研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-02-06