Contributions of Cytogenetics and Molecular Genetics to the Diagnosis and Treatment of Malignant Soft Tissue Tumors

• Project/Area Number: 21791424
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Fukuoka University
• Principal Investigator: NISHIO Jun Fukuoka University, 医学部, 助教 (90360304)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 軟部腫瘍 / 悪性線維性組織球腫 / 脂肪肉腫 / 色素性絨毛結節性滑膜炎 / 細胞株 / 遺伝子 / 分子病理学 / 生物学 / 染色体 / FISH / 肉腫 / 分子生物学 / 分子標的 / 染色体異常

Research Abstract

We established a new permanent human malignant fibrous histiocytoma cell line. Molecular cytogenetic analyses revealed a homozygous deletion of p16 and a high-level amplification of 9q31-34 in this cell line. CSFJ rearrangement was identified in about 60% of pigmented villonodular synovitis/giant cell tumor of tendon sheath cases. Also, the presence of CSFIR amplification was not identified in any cases examined. High-level amplification of 12q13-15 was found in well-differentiated liposarcomas and dedifferentiated liposarcomas but not in benign adipocytic tumors.

Research Products

• [Journal Article] Contributions of cytogenetics and molecular cytogenetics to the diagnosis of adipocytic tumors. (2011)
  • Author(s): Nishio J.
  • Journal Title: J Biomed Hiotechnol 2011 Pages: 524067-524067
  • Peer Reviewed
• [Journal Article] A novel t(4 ; 22)(q31 ; q12) produces an EWSR1-SMARCA5 fusion in extraskeletal Ewing sarcoma/primitive neuroectodermal tumor. (2011)
  • Author(s): Sumegi J, Nishio J, Nelson M, Frayer RW, Perry D, Bridge JA.
  • Journal Title: Mod Pathol 24(3) Pages: 333-342
  • Peer Reviewed
• [Journal Article] Contributions of cytogenetics and molecular cytogenetics to the diagnosis of adipocytic tumors. (2011)
  • Author(s): 西尾淳
  • Journal Title: J Biomed Biotechnol
  • Peer Reviewed
• [Journal Article] Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2 : molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization. (2010)
  • Author(s): Nishio J, Iwasaki H, Nabeshima K, Ishiguro M, Isayama T, Naito M.
  • Journal Title: J Exp Clin Cancer Res 29 Pages: 153-153
  • Peer Reviewed
• [Journal Article] Non-traumatic myositis ossificans mimicking a malignant neoplasm in an 83-year-old woman : a case report. (2010)
  • Author(s): Nishio J, Nabeshima K, Iwasaki H, Naito M.
  • Journal Title: J Med Case Reports 4 Pages: 270-270
  • Peer Reviewed
• [Journal Article] Serum CA 125 expression as a tumor marker for diagnosis and monitoring the clinical course of epithelioid sarcoma. (2010)
  • Author(s): Hoshino M, Kawashima H, Ogose A, Kudo N, Ariizumi T, Hotta T, Umezu H, Hatano H, Morita T, Nishio J, Iwasaki H, Endo N.
  • Journal Title: J Cancer Res Clin Oncol 136(3) Pages: 457-464
  • Peer Reviewed
• [Journal Article] Non-traumatic myositis ossificans mimicking a malignant neoplasm in an 83-year-old woman : a case report. (2010)
  • Author(s): 西尾淳
  • Journal Title: J Med Case Reports Volume: 4
  • Peer Reviewed
• [Journal Article] Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2 : molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization. (2010)
  • Author(s): 西尾淳
  • Journal Title: J Exp Clin Cancer Res Volume: 29
  • Peer Reviewed
• [Journal Article] Aberrations of 6q13 mapped to the COL12A1 locus in chondromyxoid fibroma. (2009)
  • Author(s): Yasuda T, Nishio J, Sumegi J, Kapels KM, Althof PA, Sawyer JR, Reith JD, Bridge JA.
  • Journal Title: Mod Pathol 22(11) Pages: 1499-1506
  • Peer Reviewed
• [Journal Article] Pathology of soft-tissue tumors : daily diagnosis, molecular cytogenetics and experimental approach. (2009)
  • Author(s): Iwasaki H, Nabeshima K, Nishio J, Jimi S, Aoki M, Koga K, Hamasaki M, Hayashi H, Mogi A.
  • Journal Title: Pathol Int 59(8) Pages: 501-521
  • Peer Reviewed
• [Journal Article] Elastofibroma dorsi : diagnostic and therapeutic algorithm.
  • Author(s): Nishio J, Isayama T, Iwasaki H, Naito M.
  • Journal Title: J Shoulder Elbow Surg in press.
  • Peer Reviewed
• [Presentation] Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2 : molecular cycogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization. (2010)
  • Author(s): Nishio J, Iwasaki H, Nabeshima K, Ishiguro M, Isayama T, Naito M.
  • Organizer: The Combined Conference of 16th Triennial Congress of Asia Pacific Orthopaedic Association and 59th Annual Meeting of Taiwan Orthopaedic Association
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-11-06
• [Presentation] Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2 : molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization. (2010)
  • Author(s): 西尾淳
  • Organizer: 16th Triennial Congress of the Asia Pacific Orthopaedic Association
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-11-06
• [Presentation] 分子機構に基づく色素性絨毛結節性滑膜炎/腱鞘巨細胞腫の診断と治療戦略 (2010)
  • Author(s): 西尾淳、内藤正俊、Julia Bridge
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-16
• [Presentation] 骨髄由来間葉系幹細胞の肉腫発生における関与について (2010)
  • Author(s): 自見至郎、鍋島一樹、青木光希子、濱崎慎、西尾淳、岩崎宏
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-15
• [Presentation] 悪性末梢神経鞘腫瘍(MPNST)におけるPDG-FR-βの発現とimatinib mesylate(Gleevec)による治療の可能性について (2010)
  • Author(s): 大石純、青木光希子、岩崎宏、鍋島一樹、古賀佳織、西尾淳、生越章、竹内保、箱崎道之
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-15
• [Presentation] 足関節に発生した粘液型脂肪肉腫の治療経験 (2010)
  • Author(s): 西尾淳、諫山照刀、吉村一朗、大慈弥裕之、岩崎宏、内藤正俊
  • Organizer: 第119回西日本・災害外科学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-06-06
• [Presentation] Tenosynovial giant cell tumor におけるCSF1再構成とCSF1R増幅の解析とその意義 (2009)
  • Author(s): 西尾淳
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-11-06
• [Presentation] 軟骨粘液線維腫の染色体異常とFISH法を川いた分子細胞遺伝学的検討 (2009)
  • Author(s): 安田剛敏、西尾淳、金森昌彦、堀岳史、鈴木賀代、木村友厚、Juha Bridge
  • Organizer: 第42回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-17
• [Presentation] 軟部腫瘍におけるテロメラーゼによらないテロメア安定機構 (2009)
  • Author(s): 石黒昌子、岩崎宏、西尾淳、濱田義浩、濱崎慎、青木光希子、鍋島一樹、平野公一、川本研一郎、原田泰志、池田丈明、茂木愛、松島悠、林博之、三宅徹
  • Organizer: 第42回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-17