Impact of anesthetic-induced preconditioning on mitochondrial ion channels
| Project/Area Number |
21791427
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
TAMPO Akihito Asahikawa Medical College, 医学部, 助教 (80531524)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 蘇生学 / プレコンディショニング / ミトコンドリア / イオンチャネル / マイトプラスト |
|---|
| Research Abstract |
We studied electrophysiological profiles of mitochondrial ion channels, which are known as important factors in anesthetic-induced preconditioning. Mytoplasts were obtained from isolated rat hearts by osmotic shock. Mitochondrial KATP channel were not detected in this study. We identified a mitochondrial ion channel with a conductance of 110pS in equimolar 150mM KCL solution. This channel current was removed when the 150mM KCL bath solution was replaced with 150mM K-glutamate. When 150mM TEA-Cl was used as bath solution, the current was not affected. In addition, The current was inhibited by DIDS, a chloride channel blocker. These results suggested that the mitochondrial ion channel was IMAC (inner membrane anion channel).
|
Report
(3 results)
Research Products
(7 results)
