Development of new gene knockdown techniques for platelets using miRNA

Research Project

Project/Area Number	21791466
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Anesthesiology/Resuscitation studies
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	KATO Yuko Kyoto Prefectural University of Medicine, 医学部附属病院, 専攻医 (50398400)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	周術期管理学 / 遺伝子治療 / 血小板
Research Abstract	After transfection of miR RNAi expression vector in human CD34+ progenitor cells and mouse bone marrow cells along with differentiating into platelets with 3-4week culture in vitro, we have confirmed the target gene knocked down in platelet cells. These platelets have less ability of aggregation and P-selectin expression in response to agonists. With injection of these gene knocked down platelets, survival and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo detailed analyses.

Report

(3 results)