| Project/Area Number |
21791477
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Juntendo University |
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Principal Investigator |
KUDO Osamu 順天堂大学, 医学部, 助教 (80365600)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 吸入麻酔薬 / 麻酔興奮期 / MEPモニタリング / セボフルラン / 電気生理 / IPSC / EPSC / 線状体 / 興奮期 / マウス / 線条体 / 発火パターン / エンケファリン / オピオイド受容体 |
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| Research Abstract |
Suppression of movement during induction of anesthesia is mediated through subcortical structures. We studied the effects of a brief 5-min application of a clinically relevant concentration of sevoflurane(2MAC : minimum alveolar concentration) on the electrophysiological activities of the medium spiny neurons of the stratum in brain slice preparations, using a whole-cell patch-clamp technique. We found that sevoflurane slightly depolarized principal neurons in the cortex and the stratum without a significant alteration in spike threshold. Furthermore, it depressed the peak, as well as the net, charge transfer of intrastriatally evoked inhibitory postsynaptic currents much more strongly than those of excitatory postsynaptic currents. And this inhibition was accompanied by an elevated paired-pulse ratio. The strong suppression of eIPSCs paralleled a significant suppression of the spontaneous EPSCs.
These results suggest that, in contrast to its effects on other brain structures, sevoflurane shifts the balance between synaptic excitation and inhibition in the direction of excitation in the stratum, thereby causing involuntary movements during induction of anesthesia by sevoflurane.
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