Analysis of prostate carcinogenesis using in vitro three-dimensional culture assay

• Project/Area Number: 21791510
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Kyushu University
• Principal Investigator: INOKUCHI Junichi 九州大学, 大学病院, 助教 (10403924)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腫瘍学 / 前立腺癌 / 発癌 / 3次元培養 / 癌 / シグナル伝達 / マイクロアレイ / 前立腺

Research Abstract

The mechanism of prostate carcinogenesis is not elucidated yet. In this study, we established a new experimental procedure for analyzing prostate carcinogenesis using in vitro three dimensional culture(3D) assays. Using this method, we showed a direct role for decreased annexin A1 expression in prostate carcinogenesis via the up-regulation of IL-6. Furthermore, we indicated an important role for androgen receptor(AR) in forming normal acinar structure in 3D culture. We reported several mechanisms of prostate carcinogenesis by regulating AR expression and its activity.

Research Products

• [Journal Article] Downregulation of phosphodiesterase 4B(PDE4B) activates protein kinase A and contributes to the progression of prostate cancer (2012)
  • Author(s): Kashiwagi E, Shiota M, Yokomizo A, Itsumi M, Inokuchi J, Uchiumi T, Naito S.
  • Journal Title: Prostate Volume: 72(7) Pages: 741-51
  • Peer Reviewed
• [Journal Article] Downregulation of phosphodiesterase 4B (PDE4B) activates protein kinase A and contributes to the progression of prostate cancer (2012)
  • Author(s): Kashiwagi E, Shiota M, Yokomizo A, Itsumi M, Inokuchi J, Uchiumi T, Naito S
  • Journal Title: Prostate Volume: 72 Pages: 741-751
  • Peer Reviewed
• [Journal Article] Statins reduce the androgen sensitivity and cell proliferation by decreasing the androgen receptor protein in prostate cancer cells (2011)
  • Author(s): Yokomizo A, Shiota M, Kashiwagi E, Kuroiwa K, Tatsugami K, Inokuchi J, Takeuchi A, Naito S.
  • Journal Title: Prostate Volume: 71(3) Pages: 298-304
  • Peer Reviewed
• [Journal Article] Shiota M, Yokomizo A, Masubuchi D, Tada Y, Inokuchi J, Eto M, Uchiumi T, Fujimoto N, Naito S. (2010)
  • Author(s): Shiota M, Yokomizo A, Masubuchi D, Tada Y, Inokuchi J, Eto M, Uchiumi T, Fujimoto N, Naito S.
  • Journal Title: Prostate Volume: 70(5) Pages: 540-54
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor gamma coactivator-1alpha interacts with the androgen receptor(AR) and promotes prostate cancer cell growth by activating the AR (2010)
  • Author(s): Shiota M, Yokomizo A, Tada Y, Inokuchi J, Tatsugami K, Kuroiwa K, Uchiumi T, Fujimoto N, Seki N, Naito S.
  • Journal Title: Mol Endocrinol Volume: 24(1) Pages: 114-27
  • Peer Reviewed
• [Journal Article] Tip60 promotes prostate cancer cell proliferation by translocation of androgen receptor into the nucleus. (2010)
  • Author(s): Shiota M, et al.
  • Journal Title: Prostate Volume: 70 Pages: 540-545
  • Peer Reviewed
• [Journal Article] Statins reduce the androgen sensitivity and cell proliferation by decreasing the androgen receptor protein in prostate cancer cells. (2010)
  • Author(s): Yokomizo A, et al.
  • Journal Title: Prostate Volume: 71 Pages: 298-304
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor gamma coactivator-lalpha interacts with the androgen receptor (AR)and promotes prostate cancer cell growth by activating the AR. (2010)
  • Author(s): Shiota M, et al.
  • Journal Title: Molecular endocrinology 24 Pages: 114-127
  • Peer Reviewed
• [Journal Article] Tip60 promotes prostate cancer cell proliferation by translocation of and rogen receptor into the nucleus. (2010)
  • Author(s): Shiota M, et al.
  • Journal Title: Prostate 70 Pages: 540-554
  • Peer Reviewed
• [Journal Article] Tyson DR, Ornstein DK. Periprostatic adipose tissue as a modulator of prostate cancer aggressiveness (2009)
  • Author(s): Finley DS, Calvert VS, Inokuchi J, Lau A, Narula N, Petricoin EF, Zaldivar F, Santos R
  • Journal Title: J Urol Volume: 182(4) Pages: 1621-7
  • Peer Reviewed
• [Journal Article] Tyson DR, Ornstein DK. Loss of annexin A1 disrupts normal prostate glandular structure by inducing autocrine IL-6 signaling (2009)
  • Author(s): Inokuchi J, Lau A
  • Journal Title: Carcinogenesis Volume: 30(7) Pages: 1082-8
  • Peer Reviewed
• [Journal Article] Loss of annexin A1 disrupts normal prostate glandular structure by ind ucing autocrine IL-6 signaling (2009)
  • Author(s): Inokuchi J, et al.
  • Journal Title: Carcinogenesis 30 Pages: 1082-1088
  • Peer Reviewed
• [Journal Article] Periprostatic adipose tissue as a modulator of prostate cancer aggressiveness. (2009)
  • Author(s): Finley DS, et al.
  • Journal Title: Journal of Urology 182 Pages: 1621-1627
  • Peer Reviewed
• [Presentation] PDE4Bの低下はPKA pathwayを活性化させ前立腺癌の進展に寄与する (2011)
  • Author(s): 柏木英志ほか
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] Prostaglandin receptor EP3 mediates growth inhibitory effect of aspirin through androgen receptor downregulation (2011)
  • Author(s): Kashiwagi E, et al
  • Organizer: 28th Korea-Japan Urological Congress
  • Place of Presentation: Suwon, Korea
  • Year and Date: 2011-09-16
• [Presentation] Peroxisome proliferator-activated receptor coactivator-1 interacts with the androgen receptor(AR) and promotes prostate cancer cell growth by activating the AR (2011)
  • Author(s): Yokomizo A, et al
  • Organizer: 106th Annual Meeting of the American Urological Association(AUA)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-05-15
• [Presentation] Peroxisome proliferator-activated receptor coactivator-1 interacts with the androgen receptor (AR) and promotes prostate cancer cell growth by activating the AR (2011)
  • Author(s): Yokomizo A, et al
  • Organizer: 106th Annual Meeting of the American Urological Association (AUA)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-05-15
• [Presentation] 前立腺がんとサイトカイン (2011)
  • Author(s): 猪口淳一、内藤誠二
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-04-22
• [Presentation] 前立腺がんとサイトカイン (2011)
  • Author(s): 猪口淳一、内藤誠二
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(招待講演)
  • Year and Date: 2011-04-22
• [Presentation] Statins reduce the androgen sensitivity and cell proliferation by decreasing the androgen receptor protein in prostate cancer cells (2011)
  • Author(s): Yokomizo A, et al
  • Organizer: 26th Annual Congress of the European Association of Urology(EAU)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-03-21
• [Presentation] 、Loss of annexin A1 disrupts normal prostate glandular structure by inducing autocrine IL-6 signaling (2010)
  • Author(s): 猪口淳一、内藤誠二
  • Organizer: 第3回前立腺生物学シンポジウム伊勢志摩2010
  • Place of Presentation: 鳥羽国際ホテル
  • Year and Date: 2010-06-18