Effect of androgen receptor on apoptosis induction in androgen- independent prostate cancer cells

• Project/Area Number: 21791514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: IGUCHI Kazuhiro Gifu Pharmaceutical University, 薬学部, 助教 (10295545)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: LNCaP細胞 / LNCaP-E9細胞 / AIDL細胞 / アンドロゲン受容体 / アポトーシス / アンドロゲン感受性 / アンドロゲン不応性 / PI3K-Akt

Research Abstract

Low-androgen-sensitive LNCaP-E9 cells and androgen-independent AIDL cells were established from androgen receptor-positive prostate cancer LNCaP cells. Androgen receptor was expressed in LNCaP-E9 and AIDL cells. Low androgen sensitivity in LNCaP-E9 cells was associated with reduced Akt phosphorylation. W741C and T877A mutations were found in AIDL cells and the mutations may explain the androgen resistance of AIDL cells.

Research Products

• [Journal Article] Castration-and aging-induced changes in the expression of zinc transporter and metallothionein in rat prostate. (2011)
  • Author(s): Iguchi, K., Morihara, N., Usui, S., Hayama, M., Sugimura, Y., Hirano, K.
  • Journal Title: J.Androl. 32 Pages: 144-150
  • Peer Reviewed
• [Journal Article] Pamidronate inhibits anti-apoptotic bcl-2 expression through inhibition of the mevalonate pathway in prostate cancer PC-3 cells. (2010)
  • Author(s): Iguchi, K., Tatsuda, Y., Usui, S., Hirano, K.
  • Journal Title: Eur.J.Pharmacol. 641 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Protein kinase C is inhibited by bisphosphonates in prostate cancer PC-3 cells. (2010)
  • Author(s): Tatsuda, Y., Iguchi, K., Usui, S., Suzui, M., Hirano, K.
  • Journal Title: Eur.J.Pharmacol. 627 Pages: 348-353
  • Peer Reviewed
• [Journal Article] Evidence that androgen-independent stromal growth factor signals promote androgen -insensitive prostate cancer cell growth in vivo. (2009)
  • Author(s): Ishii, K., Imamura, T., Iguchi, K., Arase, S., Yoshio, Y., Arima, K., Hirano, K., Sugimura, Y.
  • Journal Title: Endocr.Relat.Cancer 16 Pages: 415-428
  • Peer Reviewed
• [Journal Article] Overexpression of thymosin beta4 increases pseudopodia formation in LNCaP prostate cancer cells. (2009)
  • Author(s): Ito, M., Iguchi, K., Usui, S., Hirano, K.
  • Journal Title: Biol.Pharm.Bull. 32 Pages: 1101-1104
  • NAID: 130000117195
  • Peer Reviewed
• [Presentation] Characterization of androgen-low sensitive LNCaP subline, LNCaP-E9 cells (2010)
  • Author(s): Kazuhiro Iguchi, Kazuhiro Fukami, Kenichiro Ishii, Shigeyuki Usui, Yoshiki Sugimura, Kazuyuki Hirano
  • Organizer: International Society for Onco- developmental Biology and Medicine (ISOBM)
  • Place of Presentation: Munich, Germany.
• [Presentation] Characterization of androgen-low sensitive LNCaP subline, LNCaP-E9 cells (2010)
  • Author(s): K.Iguchi, K.Fukami, K.Ishii, S.Usui, Y.Sugimura, K.Hirano
  • Organizer: International Society for Onco-developmental Biology and Medicine (ISOBM2010)
  • Place of Presentation: ドイツ・ミュンヘン