Analysis of the cell cycle in infected culture cell of Neisseria gonorrhoeae.

• Project/Area Number: 21791535
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SHIMUTA Ken National Institute of Infectious Diseases, 細菌第一部, 主任研究官 (40370960)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 淋菌感染症 / 細胞周期 / 尿路感染症

Research Abstract

In this study, I confirmed the universality of G1 arrest caused by gonococcus infection in culture cell. I examined about the G1 arrest ability of various strains varied by MLST analysis. The host cell used HeLa cell. In the strain of 40 different phylesis, there was not the strains which significantly caused G1 arrest. Significant difference slightly examined a quantitative alteration of the cytokine production about approved two strains, but the significant difference between the strains was not recognized. These results indicated that the possibility that the G1 arrest in the gonococcus infection cell was caused by the certain strain which was not a general phenomenon.

Research Products

• [Journal Article] Temporal and spatial expression patterns of Cdc25 phosphatase isoforms during early Xenopus development (2011)
  • Author(s): Nakajo, N, Deno, Y, Ueno, H, Kenmochi, C, Shimuta, K, Sagata, N
  • Journal Title: International Journal of Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?-Detailed characterization of the first high-level ceftriaxone resistant strain. (2011)
  • Author(s): Ohnishi, M, Golparian, D, Shimuta, K, Saika, T, Hoshina, S, Iwasaku, K, Nakayama, S, Kitawaki, J, Unemo, M
  • Journal Title: Antimicrob Agents Chemother (in press)
  • Peer Reviewed
• [Journal Article] Identification of TEM-135 β-Lactamase in Penicillinase-Producing Neisseria gonorrhoeae Strains in Japan. (2010)
  • Author(s): Ohnishi, M, Ono, E, Shimuta, K, Watanabe, H, Okamura, N
  • Journal Title: Antimicrob Agents Chemother 54(7) Pages: 3021-3023
  • Peer Reviewed
• [Journal Article] Spreading of a chromosomal cefixime resistant penA gene among different Neisseria gonorrhoeae lineages. (2010)
  • Author(s): Ohnishi, M, Watanabe, Y, Ono, E, Takahashi, C, Oya, H, Kuroki, T, Shimuta, K, Okazaki, N, Nakayama, S, Watanabe, H
  • Journal Title: Antimicrob Agents Chemother 54(3) Pages: 1060-1067
  • Peer Reviewed
• [Journal Article] Spread of a Chromosomal Cefixime-Resistant penA Gene among Different Neisseria gonorrhoeae Lineages (2010)
  • Author(s): Ohnishi M, Watanabe Y, Ono E, Takahashi H, Oya H, Kuroki T, Shimuta K, Okazaki N, Nakayama S, Watanabe H
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 54 Pages: 1060-1067
  • Peer Reviewed
• [Journal Article] Identification of TEM-135 β-Lactamase in Penicillinase-Producing Neisseria gonorrhoeae Strains in Japan (2010)
  • Author(s): Ohnishi M, Ono E, shimuta k, Watanabe H, Okamura N
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 54 Pages: 3021-3023
  • Peer Reviewed
• [Presentation] 簡易的な淋菌のスクリーニング系の検討 (2010)
  • Author(s): 志牟田健
  • Organizer: 日本性感染症学会第23回学術大会
  • Place of Presentation: 福岡市
  • Year and Date: 2010-12-11
• [Presentation] 簡易的な淋菌のスクリーニング系の検討 (2010)
  • Author(s): 志牟田健、中山周一、飛田収一、伊東三喜雄、藤原光文、大前利市、石川和弘、大西真
  • Organizer: 日本性感染症学会
  • Place of Presentation: 福岡市
• [Remarks] ホームページ等
  • URL: http://www.nih.go.jp/niid/