Expression of miRNA in human epithelial ovarian cancer

• Project/Area Number: 21791537
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: AKAHIRA Junichi 東北大学, 大学院・医学系研究科, 非常勤講師 (90359505)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 婦人科腫瘍学 / 卵巣癌 / マイクロRNA / 子宮体癌

Research Abstract

miR-34b was found to have promoter hypermethylation, which when reversed, restored miR-34b expression in the endometrial serous cancer (ESC)cell lines treated with 5-aza-2' deoxycytidine. miR-34b transfection inhibited ESC cell growth, migration and most notably invasion. These effects were mediated by miR-34b target gene, the mesenchymal epithelial transition factor (MET). The expression of MET was reduced following the restoration of miR-34b in ESC cell lines. In summary, our data suggest that miR-34b plays a role in the molecular pathogenesis of endometrial cancer.

Research Products

• [Journal Article] MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma (2012)
  • Author(s): Hiroki E, Suzuki F, Akahira JI, Nagase S, Ito K, Sugawara JI, Miki Y, Suzuki T, Sasano H, Yaegashi N
  • Journal Title: Int J Cancer Volume: (in press)
  • Peer Reviewed
• [Journal Article] MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma (2012)
  • Author(s): Hiroki E, Akahira J, et al
  • Journal Title: Int J Cancer Volume: (印刷中) Issue: 4 Pages: 395-404
  • DOI: 10.1002/ijc.27345
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor-gamma suppresses CYP11B2 expression and aldosterone production (2011)
  • Author(s): Uruno A, Akahira J, et al
  • Journal Title: J Mol Endocrinol Volume: 46 Pages: 37-49
  • Peer Reviewed
• [Journal Article] Changes in microRNA expression levels correlate with clinicopathological features and prognoses in endometrial serous adenocarcinomas (2010)
  • Author(s): Hiroki E, Akahira J, Suzuki F, Nagase S, Ito K, Suzuki T, Sasano H, Yaegashi N
  • Journal Title: Cancer Sci Volume: 101(1) Pages: 241-249
  • NAID: 10027200423
  • Peer Reviewed
• [Journal Article] Steroid and Xenobiotic Receptor (SXR)as a possible prognostic marker in epithelial ovarian cancer (2010)
  • Author(s): Yue X, Akahira J, Utsunomiya H, Miki Y, Takahashi N, Niikura H, Ito K, Sasano H, Okamura K, Yaegashi N
  • Journal Title: Pathol Int Volume: 60(5) Pages: 400-406
  • NAID: 10029331942
  • Peer Reviewed
• [Journal Article] Steroid and xenobiotic receptor (SXR) as a possible prognostic marker in epithelial ovarian cancer. (2010)
  • Author(s): Yue X, Akahira J, et al.
  • Journal Title: Pathol Int Volume: 60 Pages: 400-406
  • NAID: 10029331942
  • Peer Reviewed
• [Journal Article] Immunolocalization of estrogen-producing and metabolizing enzymes inbenign breast disease : comparison with normal breast and breast carcinoma. (2010)
  • Author(s): Sasaki Y, Akahira J, et al.
  • Journal Title: Cancer Sci Volume: 101 Pages: 2286-2292
  • Peer Reviewed
• [Journal Article] Intratumoral localization of aromatase and interaction between stromal and parenchymal cells in the non-small cell lung carcinoma microenvironment. (2010)
  • Author(s): Miki Y, Akahira J, et al.
  • Journal Title: Cancer Res Volume: 70 Pages: 6659-6669
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of 17beta-hydroxysteroid dehydrogenase type 12 by SREBP-1 (2009)
  • Author(s): Nagasaki S, Akahira J, et al.
  • Journal Title: Mol Cell Endocrinol 307 Pages: 163-168
  • Peer Reviewed
• [Journal Article] Development of the human adrenal zona reticularis : morphometric and immunohistochemical studies from birth to adolescence (2009)
  • Author(s): Hui XG, Akahira J, et al.
  • Journal Title: J Endocrinol 203 Pages: 241-252
  • Peer Reviewed
• [Journal Article] Changes in microRNA expression levels correlate with clinicopathological features and prognoses in endometrial serous adenocarcinomas (2009)
  • Author(s): Hiroki E, Akahira J, et al.
  • Journal Title: Cancer Sci 101 Pages: 241-249
  • NAID: 10027200423
  • Peer Reviewed
• [Presentation] Aberrant expression and function of miR-34b in uterine papillary serous carcinoma (UPSC) (2011)
  • Author(s): 鈴木史彦、赤平純一、廣木恵理、永瀬智、伊藤潔、宇都宮裕貴、笹野公伸、八重樫伸生
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] Aberrant expression and function of miR-34b in uterine papillary serous carcinoma (UPSC) (2011)
  • Author(s): 鈴木史彦、赤平純一, ほか
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] 子宮癌肉腫, 平滑筋肉腫と内膜間質肉腫におけるSteroid and Xenobiotic Receptor (SXR)の発現と臨床病理学的因子との関連についての検討 (2011)
  • Author(s): 楽暁ジ, 赤平純一, 宇都宮裕貴, 鈴木史彦, 志賀尚美, 伊藤潔, 笹野公伸, 八重樫伸生
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2011-08-30
• [Presentation] MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma (2010)
  • Author(s): 廣木恵理、鈴木史彦、赤平純一、永瀬智、伊藤潔、笹野公伸、八重樫伸生
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] Steroid and xenobiotic receptor (SXR) as a possible prognostic marker in epithelial ovarian cancer. (2010)
  • Author(s): 廣木恵理、赤平純一, ほか
  • Organizer: 第69回日本癌学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] Role of micro-RNA in uterine papillary serous carcinoma (UPSC) (2010)
  • Author(s): 鈴木史彦、赤平純一、廣木恵理、永瀬智、伊藤潔、笹野公伸、八重樫伸生
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] 卵巣癌におけるsteroid and xenobiotic receptorの発現と臨床病理学的因子との関連についての検討 (2010)
  • Author(s): 楽暁ジ, 赤平純一, 宇都宮裕貴, 新倉仁, 伊藤潔, 笹野公伸, 八重樫伸生
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-24
• [Presentation] 子宮体部漿液性腺癌におけるマイクロRNA-34bの遺伝子発現異常およびその機能の検討 (2010)
  • Author(s): 廣木恵理, 赤平純一, 鈴木史彦, 永瀬智, 伊藤潔, 八重樫伸生
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-23
• [Presentation] 子宮体部漿液性腺癌おけるマイクロRNAの役割 (2010)
  • Author(s): 鈴木史彦, 赤平純一, 廣木恵理, 永瀬智, 伊藤潔, 笹野公伸, 八重樫伸生
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-23