| Project/Area Number |
21791548
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMAMOTO Eiko Nagoya University, 医学部附属病院, 助教 (10432262)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胎盤 / Extravillous trophoblast / 糖転移酵素 / GnT-V / 浸潤 / extravillous trophoblast / integrin alfa5beta1 / EVT / TGF-β |
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| Research Abstract |
N-acetylglucosaminyltransferase V (GnT-V) is expressed in extravillous trophoblast (EVT) of human placenta in the first-trimester. We investigated factors which regulate expression of GnT-V in EVT cells using EVT cell line, HTR-8/SVneo. The supernatant of decidual tissue culture made GnT-V expression reduced and we confirmed TGF-b was the factor in cytokines. Hypoxia (1% Oxygen) promoted GnT-V expression in mRNA and protein levels after incubation for 48 hours compared to normoxia. Because GnT-V regulates trophoblast invasion by glycosylation of integrina5b1, we investigated the affection on phosphorylation of FAK after incubation with fibronectin. The result was that phosphorylation of FAK was reduced in GnT-V shRNA transfectants copared to control cells. We also investigated the difference of GnT-V expression between normal placentas and pregnancy-induced hypertension's ones, but there was no difference in immunohistochemistry and Western blot.
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