Regulation of trophoblast invasion by N-acetylglucosaminyltransferase in human placenta
| Project/Area Number |
21791548
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMAMOTO Eiko Nagoya University, 医学部附属病院, 助教 (10432262)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胎盤 / Extravillous trophoblast / 糖転移酵素 / GnT-V / 浸潤 / extravillous trophoblast / integrin alfa5beta1 / EVT / TGF-β |
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| Research Abstract |
N-acetylglucosaminyltransferase V (GnT-V) is expressed in extravillous trophoblast (EVT) of human placenta in the first-trimester. We investigated factors which regulate expression of GnT-V in EVT cells using EVT cell line, HTR-8/SVneo. The supernatant of decidual tissue culture made GnT-V expression reduced and we confirmed TGF-b was the factor in cytokines. Hypoxia (1% Oxygen) promoted GnT-V expression in mRNA and protein levels after incubation for 48 hours compared to normoxia. Because GnT-V regulates trophoblast invasion by glycosylation of integrina5b1, we investigated the affection on phosphorylation of FAK after incubation with fibronectin. The result was that phosphorylation of FAK was reduced in GnT-V shRNA transfectants copared to control cells. We also investigated the difference of GnT-V expression between normal placentas and pregnancy-induced hypertension's ones, but there was no difference in immunohistochemistry and Western blot.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Indoleamine 2,3-dioxygenase expression predicts impaired survival of invasive cervical cancer patients treated with radical hysterectomy.2010
Author(s)
Inaba T, Ino K, Kajiyama H, Shibata K, Yamamoto E, Kondo S, Umezu T, Nawa A, Takikawa O, Kikkawa F.
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Journal Title
Gynecol Oncol. 117(3)
Pages: 423-428
Related Report
Peer Reviewed
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