| Project/Area Number |
21791549
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
GOTO Maki Nagoya University, 医学部附属病院, 助教 (90378125)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 卵胞発育 / ヒト顆粒膜細胞 / PTEN / インスリン抵抗性 / 多嚢胞性卵巣症候群 / 早発卵巣機能不全 / ヒト黄体化顆粒膜細胞 |
|---|
| Research Abstract |
Granulosa cells proliferate and then undergo differentiation ; an inverse relationship between these processes is observed during terminal follicular growth. But this proliferation/differentiation transition is not fully understood. We show that PTEN was induced with hCG treatment in human luteinized granulosa cells. Pretreatment with hCG attenuated IGF-1-induced phosphorylation of AKT and cell proliferation. Furthermore, we assess the induction of PTEN expression with insulin treatment and effects of PTEN on IGF-I-induced granulosa cell proliferation as well as the correlation of PTEN levels with the concentration of insulin in follicular fluid in PCOS and non-PCOS patients.
|
