The new development of ovulation inductor associated with the expression of PTEN in human granulosa cells.

• Project/Area Number: 21791549
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: GOTO Maki Nagoya University, 医学部附属病院, 助教 (90378125)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 卵胞発育 / ヒト顆粒膜細胞 / PTEN / インスリン抵抗性 / 多嚢胞性卵巣症候群 / 早発卵巣機能不全 / ヒト黄体化顆粒膜細胞

Research Abstract

Granulosa cells proliferate and then undergo differentiation ; an inverse relationship between these processes is observed during terminal follicular growth. But this proliferation/differentiation transition is not fully understood. We show that PTEN was induced with hCG treatment in human luteinized granulosa cells. Pretreatment with hCG attenuated IGF-1-induced phosphorylation of AKT and cell proliferation. Furthermore, we assess the induction of PTEN expression with insulin treatment and effects of PTEN on IGF-I-induced granulosa cell proliferation as well as the correlation of PTEN levels with the concentration of insulin in follicular fluid in PCOS and non-PCOS patients.

Research Products

• [Journal Article] Assessment of the predictive value of follicular fluid insulin, leptin and adiponectin in assisted reproductive cycles. (2010)
  • Author(s): Takikawa S, Iwase A, Goto M, Harata T, Umezu T, Nakahara T, Kobayashi H, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: Gynecol Endocrinol 26(7) Pages: 494-499
  • Peer Reviewed
• [Journal Article] Assessment of the predictive value of follicular fluid insulin, leptin and adiponectin in assisted reproductive cycles (2010)
  • Author(s): Takikawa S, Iwase A, Goto M, Harata T, Umezu T, Nakahara T, Kobayashi H, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: Gynecological Endocrinology Volume: 26 Pages: 494-9
  • Peer Reviewed
• [Journal Article] IGF1-induced AKT phosphorylation and cell proliferation are suppressed with the increase in PTEN during luteinization in human granulosa cells. (2009)
  • Author(s): Goto M, Iwase A, Harata T, Takigawa S, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: Reproduction. 137(5) Pages: 835-842
  • Peer Reviewed
• [Journal Article] Insulin attenuates the insulin-like growth factor-I (IGF-I)-Akt pathway, not IGF-I-extracellularly regulated kinase pathway, in luteinized granulosa cells with an increase in PTEN. (2009)
  • Author(s): Iwase A, Goto M, Harata T, Takigawa S, Nakahara T, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: J Clin Endocrinol Metab. 94(6) Pages: 2184-2191
  • Peer Reviewed
• [Journal Article] IGF1-induced AKF phosphorylation and cell proliferation are suppressed with the increase in PTEN during luteinization in human granulosa cells. (2009)
  • Author(s): Goto M, Iwase A, Harata T, Takigawa S, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: Reproduction 137 Pages: 835-842
  • Peer Reviewed
• [Journal Article] Insulin attenuates the insulin-like growth factor-I (IGF-I)-Akt pathway, not IGF-I-extracellularly regulated kinase pathway, in luteinized granulosa cells with an increase in PTEN. (2009)
  • Author(s): Iwase A, Goto M, Harata T, Takigawa S, Nakahara T, Suzuki K, Manabe S, Kikkawa F.
  • Journal Title: Journal of Clinical Endocrinology & Metabolism 94 Pages: 2184-2191
  • Peer Reviewed
• [Presentation] Establishment of cell lines of immortalized human ovarian granulose cells (2010)
  • Author(s): S.Takikawa, A.Iwase, Y.Nagatomo, B.Bayasula, W.Hirokawa, T.Nakahara, S.Manabe, H.Kobayashi, M.Goto, K.Kikkawa
  • Organizer: 26th ANNUAK MEETING European Society of Human Reproduction and Embryology
  • Place of Presentation: イタリアFiera Roma
  • Year and Date: 2010-06-29
• [Presentation] PTENは顆粒膜細胞増殖の細胞内調節因子でありインスリンで誘導される (2009)
  • Author(s): 岩瀬明, 後藤真紀, 眞鍋修一, 鈴木恭輔, 小林浩治, 滝川幸子, 中原辰夫, 廣川和加奈, 吉川史隆
  • Organizer: 第54回日本生殖医学会学術講演会
  • Place of Presentation: 金沢市 石川県立音楽堂
  • Year and Date: 2009-11-22
• [Presentation] Insulin atennates IGF-1 induced proliferation of granulosa cells with an increase in PTEN (2009)
  • Author(s): A.Iwase, M.Goto, S.Takikawa, K.Kikkawa
  • Organizer: 65Th ANNUAL MEETING American Society for Reproductive Medicine
  • Place of Presentation: アメリカGeorgia World Congress Center Atlanta
  • Year and Date: 2009-10-17
• [Presentation] Insulin atennates IGF-1 induced proliferation of granulosa cells with an increase in PTEN (2009)
  • Author(s): A.Iwase, S.Takikawa, M.Goto, T.Harata, T.Umezu, T.Nakahara, S.Manabe, K.Suzuki, H.Kobayashi, K.Kikkawa
  • Organizer: 19Th ANNUAL MEETING the International Federation of Gynecology and Obstetrics
  • Place of Presentation: 南アフリカCape Town International Convention Centre
  • Year and Date: 2009-10-04
• [Presentation] ヒト顆粒膜細胞の不死化、遺伝子導入によるcell lineの樹立について (2009)
  • Author(s): 滝川幸子, 廣川和加奈, 岩瀬明, 中原辰夫, 小林浩治, 鈴木恭輔, 眞鍋修一, 梅津朋和, 後藤真紀,吉川史隆
  • Organizer: 第27回日本受精着床学会学術講演会
  • Place of Presentation: 京都市 国立京都国際会館
  • Year and Date: 2009-08-06
• [Presentation] インスリン、アディポネクチンは卵胞発育に影響を与えるか (2009)
  • Author(s): 滝川幸子, 岩瀬明, 中原辰夫, 小林浩治, 鈴木恭輔, 眞鍋修一, 梅津朋和, 後藤真紀,吉川史隆
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都市 国立京都国際会館
  • Year and Date: 2009-04-03
• [Remarks] ホームページ等