Establishment of molecular diagnosis of fetal/placental function
| Project/Area Number |
21791567
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MIURA Kiyonori Nagasaki University, 病院, 講師 (00363490)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 胎児・胎盤機能 / 分子評価法 / microRNA / 母体血漿 / mRNA / 妊娠 / 19q13.42 / 14q32 |
|---|
| Research Abstract |
Several placental microRNAs have been identified as pregnancy-associated molecules having the potential to be used to estimate the condition of the placenta. However, the understanding of these novel molecules is still limited. The aim of this study was to isolate and characterize pregnancy-associated microRNAs in maternal plasma.
In this study, by microarray-based screening of 723 human microRNAs, we selected 82 placenta-predominantly expressed microRNAs and identified 24 of them as pregnancy-associated microRNAs. These included 16 microRNAs clustered on 19q.13.42 and 5 microRNAs on 14q32. As the pregnancy progressed into the third trimester, the plasma concentrations of CF chromosome 19-derived microRNAs (has-miR-515-3p, has-miR-517a has-miR-517c, has-miR-518b, and has-miR-526b) increased significantly, while that of CF has-miR-323-3p on chromosome 14q32.31 showed no change (P=0.2026). These new pregnancy-associated microRNAs may be useful molecular markers for monitoring pregnancy-associated diseases, e.g.intragrowth reterdation, preeclampsia, or hydatidiform mole.
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Report
(3 results)
Research Products
(66 results)
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[Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders.2011
Author(s)
Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, Masuzaki H.
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Journal Title
Journal of Human Genetics
Volume: (in press)(掲載確定)
NAID
Related Report
Peer Reviewed
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[Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women.2011
Author(s)
Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
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Journal Title
Journal of Human Genetics
Volume: (in press)(掲載確定)
NAID
Related Report
Peer Reviewed
-
[Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology.2011
Author(s)
Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
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Journal Title
The Journal of Obstetrics and Gynaecology Research
Volume: (in press)(掲載確定)
NAID
Related Report
Peer Reviewed
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[Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders.
Author(s)
Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, Masuzaki H.
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Journal Title
Journal of Human Genetics in press.
NAID
Related Report
Peer Reviewed
-
[Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women.
Author(s)
Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
-
Journal Title
Journal of Human Genetics in press
NAID
Related Report
Peer Reviewed
-
[Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology.
Author(s)
Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
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Journal Title
The Journal of Obstetrics and Gynaecology Research in press.
NAID
Related Report
Peer Reviewed
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