Innovation of prognosis and therapeutic method for complications in pregnancy using adipokine technology

• Project/Area Number: 21791571
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nara Medical University
• Principal Investigator: NARUSE Katsuhiko Nara Medical University, 医学部, 助教 (70453165)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 脂肪細胞 / アディポネクチン / アディポサイトカイン / 妊娠 / 妊娠糖尿病 / 妊娠高血圧症 / 絨毛細胞

Research Abstract

Cytokines derived from adipocyte (adipocytokine), mainly adiponectin, were studied to clarify the effects of adipocyte on trophoblasts in normal and complicated pregnancy. The effect of free fatty acid on secretion of adiponectin was suggested in pregnancy induced hypertension. Additionally, coordinal alteration of high molecular weight adiponectin and chemokine in the course of normal pregnancy were clarified.

Research Products

• [Journal Article] Effect of tumour necrosis factor-a in combination with interferon-Y on first trimester extravilous trophoblast invasion. (2011)
  • Author(s): Otun HA, Lash GE, Innes BA, Bulmer JN, Naruse K, Hannon T, Searle RF, Robson SC.
  • Journal Title: J Reprod Immunol 88 Pages: 1-11
• [Journal Article] Effect of tumour necrosis factor-α in combination with interferon-γ on first trimester extravillous trophoblast invasion. (2011)
  • Author(s): Otun HA, Lash GE, Innes BA, Bulmer JN, Naruse K, Hannon T, Searle RF, Robson SC.
  • Journal Title: J Reprod Immunol. Volume: 88 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Fatal Factors of Clinical Manifestations and Laboratory Testing in Patients with Amniotic Fluid Embolism. (2010)
  • Author(s): Oi H, Naruse K, Noguchi T, Sado T, Kimura S, Kanayama N, Terao T, Kobayashi H.
  • Journal Title: Gynecol Obstet Invest. 70 Pages: 138-144
• [Journal Article] Secretion of cytokines by villous cytotrophoblast and extravillous trophoblast in the first trimester of human pregnancy. (2010)
  • Author(s): Naruse K, Innes BA, Bulmer JN, Robson SC, Searle RF, Lash GE.
  • Journal Title: J Reprod Immunol 86 Pages: 148-150
• [Journal Article] 妊娠とアディポネクチン～正常妊婦の血中濃度推移と脂肪細胞における基礎的検討～ (2010)
  • Author(s): 成瀬勝彦
  • Journal Title: 糟尿病と妊振 10 Pages: 32-35
• [Journal Article] アディポネクチンを指標とした妊娠糖尿病および妊娠高血圧症候群の早期予知と介入効果～インスリン抵抗性周産期疾患の減少を目指して～ (2010)
  • Author(s): 成瀬勝彦, 小林浩, 佐道俊幸, 坂田麻理子, 吉田昭三, 大野木輝
  • Journal Title: 第41回「医学研究助成」研究報告集(三井生命厚生事業団) 41 Pages: 7-9
• [Journal Article] Secretion of Angiogenic Growth Factors by Vinous Cytotrophoblast and Extravillous Trophoblast in Early Human Pregnancy. (2010)
  • Author(s): Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN.
  • Journal Title: Placenta 31 Pages: 545-548
• [Journal Article] Fatal Factors of Clinical Manifestations and Laboratory Testing in Patients with Amniotic Fluid Embolism. (2010)
  • Author(s): Oi H, Naruse K, Noguchi T, Sado T, Kimura S, Kanayama N, Terao T, Kobayashi H.
  • Journal Title: Gynecol Obstet Invest. Volume: 70 Pages: 138-144
  • Peer Reviewed
• [Journal Article] Secretion of cytokines by villous cytotrophoblast and extravillous trophoblast in the first trimester of human pregnancy. (2010)
  • Author(s): Naruse K, Innes BA, Bulmer JN, Robson SC, Searle RF, Lash GE.
  • Journal Title: J Reprod Immunol. Volume: 86 Pages: 148-150
  • Peer Reviewed
• [Journal Article] 妊娠とアディポネクチン～正常妊婦の血中濃度推移と脂肪細胞における基礎的検討～ (2010)
  • Author(s): 成瀬勝彦
  • Journal Title: 糖尿病と妊娠 Volume: 10 Pages: 32-35
• [Journal Article] アディポネクチンを指標とした妊娠糖尿病および妊娠高血圧症候群の早期予知と介入効果～インスリン抵抗性周産期疾患の減少を目指して～ (2010)
  • Author(s): 成瀬勝彦, 小林浩, 佐道俊幸, 坂田麻理子, 吉田昭三, 大野木輝
  • Journal Title: 第41回「医学研究助成」研究報告集(三井生命厚生事業団) Volume: 41 Pages: 7-9
• [Journal Article] Secretion of Angiogenic Growth Factors by Villous Cytotro phoblast and Extravillous Trophoblast in Early Human Pregnancy. (2010)
  • Author(s): Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN.
  • Journal Title: Placenta Epub Ahead of Print
  • Peer Reviewed
• [Journal Article] The urokinase plasminogen activator (uPA) system in uterine natural killer cells in the placental bed during early pregnancy. (2009)
  • Author(s): Naruse K, Lash GE, Bulmer JN, Innes BA, Otun HA, Searle RF, Robson SC.
  • Journal Title: Placenta 30 Pages: 398-404
• [Journal Article] Oxytocin Hypersensitivity in Pregnant P-LAP Deficient Mice. (2009)
  • Author(s): Ishii M, Naruse K, Hattori A, Tsujimoto M, Ishiura S, Numaguchi Y, Murohara T, Kobayashi H, Mizutani S.
  • Journal Title: Life Sci. 84 Pages: 668-672
• [Journal Article] Oxytocin Hypersensitivity in Pregnant P-LAP Deficient Mice. (2009)
  • Author(s): Ishii M, Naruse K, Hattori A, Tsujimoto M, Ishiura S, Numaguchi Y, Murohara T, Kobayashi H, Mizutani S.
  • Journal Title: Life Science 84 Pages: 668-672
  • Peer Reviewed
• [Presentation] 正常妊娠における血中炎症性サイトカインとケモカインの経時的変化 (2011)
  • Author(s): 常見泰平、成瀬勝彦、大野木輝、佐道俊幸、野口武俊、小山恵美、大井豪一、小林浩
  • Organizer: 第21回腎と妊娠研究会
  • Place of Presentation: 埼玉
  • Year and Date: 2011-03-05
• [Presentation] 妊娠中の遊離脂肪酸(FFA)変動ならびにアディポサイトカインとの連関 (2010)
  • Author(s): 成瀬勝彦、大野木輝、常見泰平、野口武俊、佐道俊幸、北中孝司、大井豪一、小林浩.
  • Organizer: 第26回日本糖尿病・妊娠学会年次学術集会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-11-27
• [Presentation] Correlation of free fatty acid and adiponectin in hypertensive pregnancy : key molecules for homeostatic inflammatory linkage. (2010)
  • Author(s): Naruse K, Onogi A, Sado T, Noguchi T, Komeda S, Koyama E, Nishioka K, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: 17th World congress of the International Society for the Study of Hypertension (ISSHP).
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-10-05
• [Presentation] Correlation of free fatty acid and adiponectin in hypertensive pregnancy : key molecules for homeostatic inflammatory linkage. (2010)
  • Author(s): Naruse K, Onogi A, Sado T, Noguchi T, Komeda S, Koyama E, Nishioka K, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: 17^<th> World congress of the International Society for the Study of Hypertension (ISSHP):一般演題より口演に選出
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-10-05
• [Presentation] 正常妊娠と妊娠高血圧症における遊離脂肪酸(FFA)とアディポネクチンの連関 (2010)
  • Author(s): 成瀬勝彦、大野木輝、小山恵美、米田聡美、西岡和弘、野口武俊、佐道俊幸、北中孝司、小林浩.
  • Organizer: 第34回日本産科婦人科栄養・代謝研究会
  • Place of Presentation: 三重
  • Year and Date: 2010-09-03
• [Presentation] Invasive activities of primary extravillous trophoblast via altered activity in low oxygen condition. (2010)
  • Author(s): Naruse K, Onogi A, Noguchi T, Sado T, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: XI International Congress of Reproductive Immunology (ICRI).
  • Place of Presentation: Palm Cove Resort, Cairns, Australia
  • Year and Date: 2010-08-15
• [Presentation] Invasive activities of primary extravillous trophoblast via altered activity in low oxygen condition (2010)
  • Author(s): Naruse K, Onogi A, Noguchi T, Sado T, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: XI International Congress of Reproductive Immunology (ICRI)
  • Place of Presentation: Palm Cove Resort, Cairns, Australia
  • Year and Date: 2010-08-15
• [Presentation] 糖尿病・重症高血圧同時合併妊娠の臨床像に関する検討 (2010)
  • Author(s): 成瀬勝彦、佐道俊幸、小山恵美、米田聡美、大野木輝、吉澤順子、野口武俊、北中孝司、小林浩.
  • Organizer: 第20回腎と妊娠研究会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-06
• [Presentation] 妊娠時のケモカイン-アディポサイトカイン・クロストーク:正常妊娠および重症妊娠高血圧症における血中Monocyte Chemoattractant Protein(MCP)-1の推移から (2009)
  • Author(s): 成瀬勝彦, 大野木輝, 佐道俊幸, 重富洋志, 伊東史学, 重光愛子, 北中孝司, 小林浩.
  • Organizer: 第24回日本生殖免疫学会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-28
• [Presentation] 正常妊娠経過中のアディポネクチンおよびインスリン抵抗性関連因子の経時的推移 (2009)
  • Author(s): 成瀬勝彦, 大野木輝, 吉田昭三, 佐道俊幸, 北中孝司, 大井豪一, 小林浩.
  • Organizer: 第25回日本糖尿病・妊娠学会年次学術集会
  • Place of Presentation: 四日市市
  • Year and Date: 2009-11-20
• [Presentation] シンポジウム「妊娠とインスリン抵抗性」～妊娠とアディポネクチン:正常妊婦の血中濃度推移と脂肪細胞における基礎的検討 (2009)
  • Author(s): 成瀬勝彦、大野木輝、吉田昭三、佐道俊幸、北中孝司、大井豪一、小林浩.
  • Organizer: 第25回日本糖尿病・妊娠学会年次学術集会
  • Place of Presentation: 四日市市
  • Year and Date: 2009-11-19
• [Presentation] Adipocyte-derived cytokines alteration for systemic inflammation in hypertensive pregnancy : Studies into matemal serum and adipocyte cell line. (2009)
  • Author(s): Naruse K, Onogi A, Sado T, Shigetomi H, Komeda S, Koyama E, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: 3rd SGI International Summit 2009.
  • Place of Presentation: Sendai, Japan.
  • Year and Date: 2009-11-12
• [Presentation] Adipocyte-derived cytokines alteration for systemic inflammation in hypertensive pregnancy : Studies into matemal serum and adipocyte cell line. (2009)
  • Author(s): Naruse K, Onogi A, Sado T, Shigetomi H, Komeda S, Koyama E, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: 3rd SGI International Summit 2009
  • Place of Presentation: Sendai, Japan.
  • Year and Date: 2009-11-12
• [Presentation] ヒト初期胎盤由来の絨毛細胞低酸素培養上清におけるサイトカイン産生 (2009)
  • Author(s): 成瀬勝彦, 大野木輝, 佐道俊幸, 吉澤順子, 重富洋志, 北中孝司, 大井豪一, 小林浩.
  • Organizer: 第17回日本胎盤学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-16
• [Presentation] Effects of Adiponectin in Differentiation, Invasion and Migration of Human Trophoblastic Cells. (2009)
  • Author(s): Onogi A, Naruse K, Shigetomi H, Yoshizawa Y, Noguchi H, Sado T, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: 15th World Congress of International Federation of Placenta Association (IFPA).
  • Place of Presentation: Adelaide, Austraria
  • Year and Date: 2009-10-06
• [Presentation] Angiopoietin-like Protein 2 (AngPTL2) in Human Maternal Serum, Amniotic Fluid and Term Placental Trophoblast in Pregnancy. (2009)
  • Author(s): Naruse K, Endo M, Onogi A, Shigetomi H, Yoshizawa Y, Sado T, Oi H, Oike Y, Kobayashi H.
  • Organizer: 15th World Congress of International Federation of Placenta Association (IFPA).
  • Place of Presentation: Adelaide, Austraria
  • Year and Date: 2009-10-06
• [Presentation] Adipocyte-derived cytokines in hypertensive pregnancy : A trigger for systemic inflammation. (2009)
  • Author(s): Naruse K, Onogi A, Sado T, Shigetomi H, Yoshida S, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: European branch of International Society for Study of Hypertension in Pregnancy (ESSHP).
  • Place of Presentation: Oxford, England.
  • Year and Date: 2009-09-10
• [Presentation] Adipocyte-derived cytokines in hypertensive pregnancy : A trigger for systemic inflammation (2009)
  • Author(s): Naruse K, Onogi A, Sado T, Shigetomi H, Yoshida S, Kitanaka T, Oi H, Kobayashi H.
  • Organizer: European branch of International Society for Study of Hypertension in Pregnancy (ESSHP).
  • Place of Presentation: Oxford, England.
  • Year and Date: 2009-09-10
• [Presentation] 妊娠糖尿病妊婦における多量体アディポネクチンの動態と治療マーカーとしての可能性 (2009)
  • Author(s): 成瀬勝彦, 植栗千陽, 重富洋志, 坂田麻理子, 佐道俊幸, 北中孝司, 大井豪一, 小林浩.
  • Organizer: 第45回日本周産期・新生児医学会学術集会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-07-14
• [Presentation] 脂肪細胞および絨毛細胞培養系におけるアディポネクチンの誘導と浸潤・分化の制御. (2009)
  • Author(s): 成瀬勝彦、大野木輝、野口武俊、坂田麻理子、佐道俊幸、北中孝司、大井豪一、小林浩.
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2009-04-05