Antitumor effect of siRNA on endometrial cancer

• Project/Area Number: 21791573
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: YANOKURA Megumi 慶應義塾大学, 医学部, 特任助教 (20433732)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: siRNA / 子宮体癌 / 分子イメージング / CHFR / タキサン製剤 / Aurora-A / in vivoイメージング

Research Abstract

Tumor development occurred 3 weeks after transplantation of HEC-1B cells in nude mice. Drugs were administered on that day and thereafter. The tumor diameter significantly decreased in mice treated with paclitaxel+Aurora-A siRNA compared with the results for other treatments(P<0. 05). The GFP fluorescence signal also markedly decreased after treatment with paclitaxel+Aurora-A siRNA.
Aurora-A siRNA may have the potential to increase the sensitivity of endometrial tumors to treatment with paclitaxel.

Research Products

• [Journal Article] Endometrial Cancer and Hypermethylation : Regulation of DNA a nd microRNA by Epigenetics (2012)
  • Author(s): Banno K, Kisu I, Yanokura M, Masuda K, Kobayashi Y, Ueki A, Tsuji K, Yamagami W, Nomura H, Susumu N, Aoki D
  • Journal Title: Biochemistry Research International Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Epigenetics and genetics in endometrial cancer : new carcinogenic mechanisms and relationship with clinical practice (2012)
  • Author(s): Banno K, Kisu I, Yanokura M, Masuda K, Ueki A, Kobayashi Y, Susumu N, Aoki D
  • Journal Title: Epigenomics Volume: 4 Pages: 147-162
  • Peer Reviewed
• [Journal Article] Epigenetic DNA hypermethylation : clinical applications in endometrial cancer(Review) (2009)
  • Author(s): Muraki Y, Banno K, Yanokura Mm, et al(6).
  • Journal Title: Oncol Rep. 22 Pages: 967-972
  • Peer Reviewed
• [Journal Article] Analysis of a correlation between the BRAF V600E mutation and abnormal DNA mismatch repair in patients with sporadic endometrial cancer (2009)
  • Author(s): Kawaguchi M, Yanokura M, Banno K, et al(7).
  • Journal Title: Int J Oncol. 34 Pages: 1541-1547
  • Peer Reviewed
• [Journal Article] Endometrial cancer as a familial tumor : pathology and molecular carcin ogenesis(review). (2009)
  • Author(s): Banno K, Yanokura M, Kobayashi Y, et al(5).
  • Journal Title: Curr Genomics. 10 Pages: 127-132
  • Peer Reviewed
• [Journal Article] Endometrial Cancer and Hypermethylation : Regulation of DNA and microRNA by Epigenetics
  • Author(s): Banno K, Kisu I, Yanokura M, Masuda K, Kobayashi Y, Ueki A, Tsuji K, Yamagami W, Nomura H, Susumu N, Aoki D
  • Journal Title: Biochemistry Research International Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Biomarkers in Endometrial Cancer : Possible clinical applications (Review)
  • Author(s): Banno K, Kisu I, Yanokura M, Tsuji K, Masuda K, Ueki A, Kobayashi Y, Yamagami W, Nomura H, Tominaga E, Susumu N, Aoki D
  • Journal Title: Oncology Letters Volume: (印刷中)
  • Peer Reviewed