Replication of HIV in immortalized human trophoblasts

• Project/Area Number: 21791575
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nihon University
• Principal Investigator: IZUMI Yasuyuki Nihon University, 医学部, 研究員 (50459872)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: HIV / 垂直感染 / 胎盤絨毛 / Trophoblast / LPS / 胎盤関門

Research Abstract

HIV replicates in immortalized human first trimester trophoblast cell line in a CD4 independent manner. The HIV replication was up-regulated by lipopolysaccharide. These results suggested molecular basis of increased risk of maternal fetal infection by local infection by Gram-negative bacteria.

Research Products

• [Journal Article] Lipopolysaccharide (LPS)-induced interferon (IFN)-gamma production by decidual mononuclear cells (DMNC) is interleukin (IL)-2 and IL-12 dependent (2011)
  • Author(s): Negishi M, Izumi Y, 他3名
  • Journal Title: American Journal of Reproductive Immunology Volume: 65 Pages: 20-27
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide(LPS)-induced Interferon(IFN)-γ production by decidual mononuclear cells is Interleukin(IL)-2 and IL-12 dependent (2010)
  • Author(s): Negishi M, Izumi Y, Aleemuzzaman S, Inaba N, Hayakawa S.
  • Journal Title: American Journal of Reproductive Immunology 2010.3.20accepted
  • Peer Reviewed
• [Journal Article] Subtyping and env C2/V3 sequence analysis of HIV-1 isolated from HIV-infected children hospitalized in Children Hospital 1, Vietnum during 2004-2005 (2009)
  • Author(s): Trinh QD, Pham NT, LamBQ, LeTP, Truong KH, Le TQ, Vo HT, Tang TC, Ha TM, Izumi Y, Mizuguchi M, Hayakawa S, Ushijima H.
  • Journal Title: Journal of Tropical Pediatrics Vol.55 Pages: 399-401
  • Peer Reviewed
• [Journal Article] H3N2 influenza A Virus replicates in immortalized human first trimester trophoblast cell lines and induces their rapid apoptosis (2009)
  • Author(s): Trinh QD, Izumi Y, Komine-Aizawa S, Shibata T, Shimotai Y, Kuroda K, Mizuguchi M, Ushijima H, Mor G, Hayakawa S.
  • Journal Title: American Journal of Reproductive Immunology Vol.62 Pages: 139-46
  • Peer Reviewed
• [Presentation] 胎盤絨毛細胞におけるHIV感染と複製の解析 (2010)
  • Author(s): 泉泰之
  • Organizer: 第84回日本感染症学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 胎盤絨毛細胞におけるHIV感染と複製の解析 (2010)
  • Author(s): 泉泰之, 他共同演者3名
  • Organizer: 第84回日本感染症学会学術講演会
  • Place of Presentation: 国立京都国際会館