Novel function of hTERT and its application to cancer therapy

• Project/Area Number: 21791579
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa University
• Principal Investigator: MAIDA Yoshiko Kanazawa University, 医学系, 助教 (20397219)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: テロメレース / 機能性RNA / RNA / テロメラーゼ / RNA依存性RNAポリメラーゼ / TERT / RMRP

Research Abstract

hTERT elongates telomere through its telomere-specific RNA dependent DNA polymerase activity. Accumulating evidence indicates hTERT has other function(s) beyond telomere maintenance. We identified RMRP as a novel binding partner of hTERT. hTERT-RMRP complex exhibited RNA dependent RNA polymerase (RdRP) activity and synthesized complementary strand of RMRP in vivo. The short RNA derived from the product of the RdRP activity was supposed to be involved in RNA silencing.

Research Products

• [Journal Article] RNA-dependent RNA polymerases in RNA silencing. (2011)
  • Author(s): Maida, Y., Masutomi, K.
  • Journal Title: Biol.Chem. 392 Pages: 299-304
  • Peer Reviewed
• [Journal Article] RNA-dependent RNA polymerase in RNA silencing (2011)
  • Author(s): Maida. Y
  • Journal Title: Biological Chemistry Volume: 392 Pages: 299-304
  • Peer Reviewed
• [Journal Article] An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA. (2009)
  • Author(s): Maida, Y., Yasukawa, M., Furuuchi, M.,Lassmann, T., Possemato, R., Okamoto,N., Kasim, V., Hayashizaki, Y., Hahn,W.C., Masutomi, K.
  • Journal Title: Nature 461 Pages: 230-235
  • Peer Reviewed
• [Journal Article] An RNA-dependent RNA polymerase formed by TERT and the RMRPRNA. (2009)
  • Author(s): Maida.Y, et al.
  • Journal Title: Nature 461 Pages: 230-235
  • Peer Reviewed
• [Presentation] hTERT produces endogenoussi RNA through its RNA-dependent RNA polymerase activity. (2009)
  • Author(s): 毎田佳子, 安川麻美, 岡本奈緒子, Vivi Kasim, Timo Lassmann, 林崎良英, 増富健吉
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] hTERT produces endogenous siRNA through its RNA-dependent RNA polymerase activity (2009)
  • Author(s): 毎田圭子
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] Involvement of hTERT in producing endogenoussi RNA through its RdRP activity. (2009)
  • Author(s): 毎田佳子, 安川麻美, 岡本奈緒子, Vivi Kasim, Timo Lassmann, 林崎良英, William C.Hahn, 増富健吉
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-10-03
• [Presentation] Involvement of hTERT in producing endogenous siRNA through its RdRP activity (2009)
  • Author(s): 毎田圭子
  • Organizer: 第68回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-10-03