Elucidation of the molecular mechanism of human life sprout

• Project/Area Number: 21791581
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: YAMADA Mitsutoshi 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 共同研究員 (40383864)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 生殖医学 / Hmgp / 着床周辺期胚発生 / RNA干渉性 / ES細胞 / 胚性遺伝子活性化 / 新規遺伝子 / 転写因子 / ECAT遺伝子 / 着床前期特異的 / siRNA / 着床周辺期発生 / RNA干渉法 / ヒト幹細胞 / 胎児発育 / 胎盤 / 胎児治療 / High mobility groupタンパク質

Research Abstract

Mining gene-expression-profiling data identified a novel gene that is specifically expressed in preimplantation embryos. Hmgpi, a putative chromosomal protein with two high-mobility-group boxes, is zygotically transcribed during zygotic genome activation, but is not transcribed postimplantation. The Hmgpi-encodedprotein(HMGPI), first detected at the 4-cell stage, remains highly expressed in pre-implantation embryos. Interestingly, HMGPI is expressed in both the inner cell mass(ICM) and the trophectoderm, and translocated from cytoplasm to nuclei at the blastocyst stage, indicating differential spatial requirements before and after the blastocyst stage. siRNA(siHmgpi)-induced reduction of Hmgpi transcript levels caused developmental loss of preimplantation embryos and implantation failures.

Research Products

• [Journal Article] Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development (2010)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Chikazawa N, Kuji N, Yoshimura Y, Umezawa A.
  • Journal Title: Human Molecular Genetics Volume: 19(3) Issue: 3 Pages: 480-493
  • DOI: 10.1093/hmg/ddp512
  • Peer Reviewed
• [Journal Article] Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development. (2010)
  • Author(s): 山田満稔、浜谷敏生, ほか
  • Journal Title: Human Molecular Genetics VOL.13 Pages: 480-493
  • Peer Reviewed
• [Journal Article] Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9. (2009)
  • Author(s): 三浦巧、阿久津英憲、山田満稔, ほか
  • Journal Title: Differentiation 78巻 Pages: 137-142
  • Peer Reviewed
• [Presentation] Age-associated alteration of telomere biology in mouse oocytes (2011)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Fukunaga T, Kuji N, Yoshimura Y
  • Organizer: American Society for Reproductive Medicine 67th Annual Meeting
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-10-19
• [Presentation] 着床前期特異的新規遺伝子Hmgpiによる着床周辺期発生の制御機構 (2011)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、近澤奈々、久慈直昭、青木大輔、吉村泰典
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-08-30
• [Presentation] 着床前期特異的新規遺伝子Hmgpiによる着床周辺期発生の制御機構 (2010)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、奥村典子、持丸佳之、浅田弘法、久慈直昭、青木大輔、吉村泰典
  • Organizer: 第51回日本哺乳動物卵子学会学術講演会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2010-05-30
• [Presentation] 着床前期特異的新規遺伝子Hmgpiによる着床周辺期発生の制御機構 (2010)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、奥村典子、持丸佳之、高野光子、浅田弘法、久慈直昭、梅澤明弘、吉村泰典
  • Organizer: 第51回日本哺乳動物卵子学会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2010-05-30
• [Presentation] 着床前期特異的新規遺伝子Hmgpによる着床周辺期発生の制御機構 (2010)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、奥村典子、持丸佳之、浅田弘法、久慈直昭、青木大輔、吉村泰典第
  • Organizer: 62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2010-05-10
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development (2010)
  • Author(s): Yamada, M, Hamatani, T, Akutsu, H, Chikazawa, N, Kuji, N, Yoshimura, Y, Umezawa, A.
  • Organizer: 1st State Key Laboratory of Reproductive Biology (SKLRB) Symposia
  • Place of Presentation: Beijing Friendship Hotel
  • Year and Date: 2010-05-10
• [Presentation] 着床前期特異的新規遺伝子Hmgpによる着床周辺期発生の制御機構 (2010)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、奥村典子、持丸佳之、浅田弘法、久慈直昭、青木大輔、吉村泰典
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2010-04-23
• [Presentation] Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development (2010)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Chikazawa N, Ogawa S, Okumura N, Mochimaru Y, Kuji N, Aoki D, Yoshimura Y, Umezawa A.
  • Organizer: The 26th Annual Meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Roma, Italy
• [Presentation] Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development (2010)
  • Author(s): Yamada M Hamatani T, Akutsu H, Chikazawa N, Kuji N, Yoshimura Y, Umezawa A.
  • Organizer: The 1st State Key Laboratory of Reproductive Biology(SKLRB) Symposia
  • Place of Presentation: Beijing, China
• [Presentation] Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development (2010)
  • Author(s): Yamada, M, Hamatani, T, Akutsu, H, Chikazawa, N, Ogawa, S, Okumura, N, Mochimaru, Y, Kuji, N, Aoki, D, Yoshimura, Y, Umezawa, A.
  • Organizer: 26^<th> Annual Meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Rome
• [Presentation] 着床前期特異的新規遺伝子Hmgpによる着床周辺期発生の制御機構 (2009)
  • Author(s): 山田満稔
  • Organizer: 第54回日本生殖医学会総会および学術講演会
  • Place of Presentation: ANAクラウンプラザホテル金沢(石川県)
  • Year and Date: 2009-11-22
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development (2009)
  • Author(s): Yamada M
  • Organizer: American Society for Reproductive Medicine〓65th Annual Meeting
  • Place of Presentation: Georgia World CongressCenter (Atlanta, Georgia, USA,)
  • Year and Date: 2009-10-21
• [Presentation] 着床前期胚に特異的に発現する新規遺伝子Hmgpの発現および機能解析 (2009)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、持丸佳之、高野光子、浅田弘法、久慈直昭、青木大輔、梅澤明弘、吉村泰典
  • Organizer: 第50回日本哺乳動物卵子学会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-05-09
• [Presentation] 着床前期胚に特異的に発現する新規遺伝子Hmgpの発現および機能解析 (2009)
  • Author(s): 山田満稔
  • Organizer: 第50回日本哺乳動物卵子学会
  • Place of Presentation: 都市センターホテル(東京都)
  • Year and Date: 2009-05-09
• [Presentation] 着床前期胚に特異的に発現する新規遺伝子Hmgpの発現および機能解析 (2009)
  • Author(s): 山田満稔、浜谷敏生、阿久津英憲、持丸佳之、高野光子、浅田弘法、久慈直昭、青木大輔、吉村泰典
  • Organizer: 第61回日本産科婦人科学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-04-03
• [Presentation] 着床前期胚に特異的に発現する新規遺伝子Hmgpの発現および機能解析 (2009)
  • Author(s): 山田満稔
  • Organizer: 第61回日本産科婦人科学会総会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-04-03
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development (2009)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Kuji N, Aoki D, Yoshimura Y.
  • Organizer: American Society for Reproductive Medicine 65th Annual Meeting
  • Place of Presentation: Atlanta, USA
• [Remarks]
  • URL: http://www.eggandstem.org/project/