| Project/Area Number |
21791592
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAYASU Yukihiro Gunma University, 医学部, 講師 (70375533)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | めまい / 虚血性神経障害 / パッチクランプ / 前庭神経核 / 脳幹 / 前庭小脳 / 虚血 |
|---|
| Research Abstract |
We tested spontaneous EPSCs (sEPSCs) and spontaneous IPSCs (sIPSCs) in neurons in the vestibular nucleus in rat acute slices of brainstem using whole-cell patch-clamp recording. sEPSCs and sIPSCs were recorded at -70mV and +30 mV of voltage-clamped membrane potentials, respectively. Pharmacological experiments have shown that sEPSCs and sIPSCs are mediated by glutamatergic and GABAergic inputs, respectively. Next, we applied Oxygen-Glucose deprivated external solution to mimic the ischemic insult. However, we did not detect any clear effects on sEPSCs in the vestibular neurons during ischemic stimulation.
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