| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Using side population method and spheroid cultivating method, we have sorted cancer stem like cell(CSC) and non-CSC from tongue cancer cell line, OSC19. We transplanted the CSC and non-CSC to tongue of nude mouse and determined frequency of neck metastasis. CSC formed more tumor of tongue and neck metastasis than non-CSC. However, non-CSC could form neck metastasis by increasing of cell the number of cells transplanted. Upon with these findings, we concluded that both CSC and non-CSC have phenotype of cancer stem cells. By analysis with immuno-histochemistry with tongue cancer tissues, CD44 and ABCG2 were determined as candidates of CSC-metastatic marker although the expression does not relate with worse prognosis. Suppression of phenotypes of CSC may control neck metastasis of tongue cancer.
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