Gene therapy of congenital hereditary hearing loss with embryonic gene transfer into the otocyst

Research Project

Project/Area Number	21791623
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Kumamoto University
Principal Investigator	MIWA Toru Kumamoto University, 医学部附属病院, 非常勤診療医師 (70535591)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords	耳科学 / 胎生期内耳 / 遺伝子導入 / 蛋白導入 / コネキシン30 / エレクトロポレーション
Research Abstract	A plamid consisting EGFP-conjugated Cx30 was transferred into the inner ear cells of the normal mice with electroporation. It was revealed that no morphological and functional changes occurred in the mice post Cx30 gene trasnsfer into the otocyst of the normal mice.