Functional analysis and differentiation induction of M cell in human nasal epithelial cells : a new treatment strategy via DDS in nasal mucosa

• Project/Area Number: 21791626
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Sapporo Medical University
• Principal Investigator: GO Mitsuru Sapporo Medical University, 医学部, 講師 (80448604)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 耳鼻咽喉学 / アレルギー / 鼻粘膜 / 病理学 / タイト結合

Research Abstract

Nasopharynx and nasal mucosa are located at the forefront of the host defense against foreign pathogens, which plays an important role in acquired immunity and innate immunity. To elucidate the immune system of allergy infection, we analyzed the mechanism of epithelial barrier function and antigen presentation in the nasal epithelium of human nasopharyngeal epithelium.

Research Products

• [Journal Article] Altered expression of claudin-1, claudin-7, and tricellulin regardless of human papilloma virus infection in human tonsillar squamous cell carcinoma. (2011)
  • Author(s): Kondoh A, Takano KI, Kojima T, Ohkuni T, Kamekura R, Ogasawara N, Go M, Sawada N, Himi T.
  • Journal Title: Acta Otolaryngol.Epub
  • Peer Reviewed
• [Journal Article] Epithelial barrier and antigen uptake in lymphoepithelium of human adenoids. (2011)
  • Author(s): Ogasawara N, Kojima T, Go M, Takano K, Kamekura R, Ohkuni T, Koizumi J, Masaki T, Fuchimoto J, Obata K, Kurose M, Shintani T, Sawada N, Himi T.
  • Journal Title: Acta Otolaryngol. 131(2) Pages: 116-123
  • Peer Reviewed
• [Journal Article] Epithelial barrier and antigen uptake in lymphoepithelium of human adenoids. (2011)
  • Author(s): Ogasawara N
  • Journal Title: Acta Otolaryngol Volume: 131 Pages: 116-123
  • Peer Reviewed
• [Journal Article] 扁桃の免疫機能と病態抗原提示経路としての扁桃上皮の役割 (2010)
  • Author(s): 小笠原徳子, 郷 充, 高野賢一, 氷見徹夫
  • Journal Title: 小児耳鼻咽喉科 31巻3号 Pages: 244-247
  • Peer Reviewed
• [Journal Article] PPARgamma agonists upregulate the barrier function of tight junctions via a PKC pathway in human nasal epithelial cells. (2010)
  • Author(s): Ogasawara N, Kojima T, Go M, Ohkuni T, Koizumi JI, Kamekura R, Masaki T, Murata M, Tanaka S, Fuchimoto J, Himi T, Sawada N.
  • Journal Title: Pharmacol Res. 61 Pages: 489-498
  • Peer Reviewed
• [Journal Article] Thymic stromal lymphopoietin induces tight junction protein claudin-7 via NF-kappaB in dendritic cells. (2010)
  • Author(s): Kamekura R, Kojima T, Takashima A, Koizumi J, Ogasawara N, Go M, Takano K, Murata M, Tanaka S, Ichimiya S, Himi T, Sawada N.
  • Journal Title: Histochem Cell Biol. 133(3) Pages: 339-348
  • Peer Reviewed
• [Journal Article] Thymic stromal lymphopoietin induces tight junction protein claudin-7 via NF-kappaB in dendritic cells. (2010)
  • Author(s): Kamekura R
  • Journal Title: Histochem Cell Biol. Volume: 133 Pages: 339-348
  • Peer Reviewed
• [Journal Article] PPARgamma agonists upregulate the barrier function of tight junctions viaa PKC pathway in human nasal epithelial cells. (2010)
  • Author(s): Ogasawara N
  • Journal Title: Pharmacol Res. Volume: 61 Pages: 489-498
  • Peer Reviewed
• [Journal Article] Thymic stromal lymphopoietin enhances tight-junction barrier function of human nasal epithelial cells. (2009)
  • Author(s): Kamekura R, Kojima T, Koizumi JI, Ogasawara N, Kurose M, Go M, Harimaya A, Murata M, Tanaka S, Chiba H, Himi T, Sawada N.
  • Journal Title: Cell Tissue Res. 338(2) Pages: 283-293
  • Peer Reviewed
• [Journal Article] Induction of JAM-A during differentiation of human THP-1 dendritic cells (2009)
  • Author(s): Ogasawara N, Kojima T, Go M, Fuchimoto J, Kamekura R, Koizumi JI, Ohkuni T, Masaki T, Murata M, Tanaka S, Ichimiya S, Himi T, Sawada N.
  • Journal Title: Biochem Biophys Res Commun. 20;389(3) Pages: 543-549
  • Peer Reviewed
• [Journal Article] Thymic stromal lymphopoietin enhances tight-junction barrier function of human nasal epithelial cells. (2009)
  • Author(s): Kamekura R
  • Journal Title: Cell Tissue Res. 338 Pages: 283-293
  • Peer Reviewed
• [Journal Article] Induction of JAM-A during differentiation of human THP-1 dendritic cells. (2009)
  • Author(s): Ogasawara N
  • Journal Title: Biochem Biophys Res Commun. 389 Pages: 543-549
  • Peer Reviewed
• [Journal Article] Ohkuni T, Kojima T, Ogasawara N, Masaki T, Ninomiya T, Kikuchi S, GoM, Takano K, Himi T, Sawada N. (2009)
  • Author(s): Ohkuni T
  • Journal Title: Med Mol Morphol. 42 Pages: 204-211
  • Peer Reviewed
• [Presentation] EXPRESSION AND FUNCTION OF TIGHT JUNCTIONS IN THE CRYPT EPITHELIUM OF HUMAN PALATINE TONSILS. (2010)
  • Author(s): Go M, Kojima T, Takano KI, Kurose M, Ohkuni T, Masaki T, Kamekura R, Kondo A, Ogasawara N, Himi T, Sawada N.
  • Organizer: 7th International Symposium on Tonsils and Mucosal Barriers of the Upper Airways.
  • Place of Presentation: Asahikawa, JAPAN
• [Presentation] EXPRESSION AND FUNCTION OF TIGHT JUNCTIONS IN THE CRYPT EPITHELIUM OF HUMAN PALATINE TONSILS (2010)
  • Author(s): Go M
  • Organizer: 7th International Symposium on Tonsils and Mucosal Barriers of the Upper Airways
  • Place of Presentation: Asahikawa, JAPAN
• [Presentation] 核内受容体PPARγを介したヒト鼻粘膜上皮細胞, 樹状細胞のタイト結合の調節機構:アレルギー性鼻炎の予防・治療のための基礎研究 (2009)
  • Author(s): 小笠原徳子, 小島 隆, 郷 充, 亀倉隆太, 高野賢一, 大國 毅, 正木智之, 澤田典均, 氷見徹夫
  • Organizer: 第27回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 千葉県
  • Year and Date: 2009-02-12