Regeneration of rabbit's esophagus proposing to pharyngeal regeneration-tissue engineering of using PGA sheet-

• Project/Area Number: 21791629
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Fukushima Medical University
• Principal Investigator: MIURA Tomohiro Fukushima Medical University, 医学部, 助教 (00423806)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 気管食道学 / PGAシート / 咽頭再生 / 食道再生

Research Abstract

This study was conducted to establish the full-thickness defect model of rabbit esophagus and to examine usability of PGA(polyglycolic acid) sheet to regenerate of rabbit esophagus. One week after the operation, a connective tissue layer formed under the PGA sheet. Two weeks after models showed the extension of the mucosal layer along that connective tissue. It was found that PGA sheet disappeared until four weeks after surgery. The smooth muscle layer regenerated in disarray in contrast to the mucosal layer reconstructing continuously. All models that treated more than 12mm horizontal defect of esophagus died. It was considered that durability of PGA sheet was not enough to regenerate in broad defect.